TY - JOUR
T1 - Micro-nano bioactive glass particles incorporated porous scaffold for promoting osteogenesis and angiogenesis in vitro
AU - Tian, Ting
AU - Xie, Weihan
AU - Gao, Wendong
AU - Wang, Gang
AU - Zeng, Lei
AU - Miao, Guohou
AU - Lei, Bo
AU - Lin, Zhanyi
AU - Chen, Xiaofeng
N1 - Publisher Copyright:
© 2019 Tian, Xie, Gao, Wang, Zeng, Miao, Lei, Lin and Chen.
PY - 2019
Y1 - 2019
N2 - Constructing the interconnected porous biomaterials scaffolds with osteogenesis and angiogenesis capacity is extremely important for efficient bone tissue engineering. Herein, we fabricated a bioactive micro-nano composite scaffolds with excellent in vitro osteogenesis and angiogenesis capacity, based on poly (lactic-co-glycolic acid) (PLGA) incorporated with micro-nano bioactive glass (MNBG). The results showed that the addition of MNBG enlarged the pore size, increased the compressive modulus (4 times improvement), enhanced the physiological stability and apatite-forming ability of porous PLGA scaffolds. The in vitro studies indicated that the PLGA-MNBG porous scaffold could enhance the mouse bone mesenchymal stem cells (mBMSCs) attachment, proliferation, and promote the expression of osteogenesis marker (ALP). Additionally, PLGA-MNBG could also support the attachment and proliferation of human umbilical vein endothelial cells (HUVECs), and significantly enhanced the expression of angiogenesis marker (CD31) of HUVECs. The as-prepared bioactive PLGA-MNBG nanocomposites scaffolds with good osteogenesis and angiogenesis probably have a promising application for bone tissue regeneration.
AB - Constructing the interconnected porous biomaterials scaffolds with osteogenesis and angiogenesis capacity is extremely important for efficient bone tissue engineering. Herein, we fabricated a bioactive micro-nano composite scaffolds with excellent in vitro osteogenesis and angiogenesis capacity, based on poly (lactic-co-glycolic acid) (PLGA) incorporated with micro-nano bioactive glass (MNBG). The results showed that the addition of MNBG enlarged the pore size, increased the compressive modulus (4 times improvement), enhanced the physiological stability and apatite-forming ability of porous PLGA scaffolds. The in vitro studies indicated that the PLGA-MNBG porous scaffold could enhance the mouse bone mesenchymal stem cells (mBMSCs) attachment, proliferation, and promote the expression of osteogenesis marker (ALP). Additionally, PLGA-MNBG could also support the attachment and proliferation of human umbilical vein endothelial cells (HUVECs), and significantly enhanced the expression of angiogenesis marker (CD31) of HUVECs. The as-prepared bioactive PLGA-MNBG nanocomposites scaffolds with good osteogenesis and angiogenesis probably have a promising application for bone tissue regeneration.
KW - Bioactive glass
KW - Bone regeneration
KW - Micro-nano particles
KW - Nanocomposites scaffolds
KW - Osteogenesis
UR - https://www.scopus.com/pages/publications/85064648235
U2 - 10.3389/fchem.2019.00186
DO - 10.3389/fchem.2019.00186
M3 - 文章
AN - SCOPUS:85064648235
SN - 2296-2646
VL - 7
JO - Frontiers in Chemistry
JF - Frontiers in Chemistry
IS - MAR
M1 - 186
ER -