Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria

Shen Li; Yuan Liu; Zixi Gao; Chao An; Hui Gu; Heng Yin; Rongzhan Fu; Liang Shi; Wenjiao Xue; Daidi Fan; Qiang Fei

doi:10.1021/acs.jafc.5c01087

Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria

Shen Li
, Yuan Liu
, Zixi Gao
, Chao An
, Hui Gu
, Heng Yin
, Rongzhan Fu
, Liang Shi
, Wenjiao Xue
, Daidi Fan
, Qiang Fei

School of Chemical Engineering and Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Bioconversion of methane into functional polysaccharides presents a promising strategy for mitigating methane-induced greenhouse gas emissions and addressing the limitations of plant-derived polysaccharide and sugar-based microbial polysaccharides production. In this study, the novel methane-derived polysaccharide (MePS) was obtained from a newly isolated methanotrophic bacterium Alkalicoccus glycogenes WONF2802. Structural characterization found that MePS is a branched-chain glucan with a weight-average molecular weight of 283.2 kDa. Additionally, MePS exhibited considerable antioxidant capacities in both in vitro biochemical assays and the H₂O₂-induced oxidative stress cell model. This work establishes a potential pathway for polysaccharide production, reducing reliance on plant and sugar-based feedstocks, while offering a new strategy for methane emission reduction.

Original language	English
Pages (from-to)	11019-11029
Number of pages	11
Journal	Journal of Agricultural and Food Chemistry
Volume	73
Issue number	18
DOIs	https://doi.org/10.1021/acs.jafc.5c01087
State	Published - 7 May 2025

Keywords

antioxidant
methane
methanotrophic bacterium
polysaccharides
structural characterization

Access to Document

10.1021/acs.jafc.5c01087

Cite this

@article{b4de0a2201964424a77e7936b79e0d04,

title = "Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria",

abstract = "Bioconversion of methane into functional polysaccharides presents a promising strategy for mitigating methane-induced greenhouse gas emissions and addressing the limitations of plant-derived polysaccharide and sugar-based microbial polysaccharides production. In this study, the novel methane-derived polysaccharide (MePS) was obtained from a newly isolated methanotrophic bacterium Alkalicoccus glycogenes WONF2802. Structural characterization found that MePS is a branched-chain glucan with a weight-average molecular weight of 283.2 kDa. Additionally, MePS exhibited considerable antioxidant capacities in both in vitro biochemical assays and the H2O2-induced oxidative stress cell model. This work establishes a potential pathway for polysaccharide production, reducing reliance on plant and sugar-based feedstocks, while offering a new strategy for methane emission reduction.",

keywords = "antioxidant, methane, methanotrophic bacterium, polysaccharides, structural characterization",

author = "Shen Li and Yuan Liu and Zixi Gao and Chao An and Hui Gu and Heng Yin and Rongzhan Fu and Liang Shi and Wenjiao Xue and Daidi Fan and Qiang Fei",

note = "Publisher Copyright: {\textcopyright} 2025 American Chemical Society.",

year = "2025",

month = may,

day = "7",

doi = "10.1021/acs.jafc.5c01087",

language = "英语",

volume = "73",

pages = "11019--11029",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "18",

}

TY - JOUR

T1 - Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria

AU - Li, Shen

AU - Liu, Yuan

AU - Gao, Zixi

AU - An, Chao

AU - Gu, Hui

AU - Yin, Heng

AU - Fu, Rongzhan

AU - Shi, Liang

AU - Xue, Wenjiao

AU - Fan, Daidi

AU - Fei, Qiang

PY - 2025/5/7

Y1 - 2025/5/7

N2 - Bioconversion of methane into functional polysaccharides presents a promising strategy for mitigating methane-induced greenhouse gas emissions and addressing the limitations of plant-derived polysaccharide and sugar-based microbial polysaccharides production. In this study, the novel methane-derived polysaccharide (MePS) was obtained from a newly isolated methanotrophic bacterium Alkalicoccus glycogenes WONF2802. Structural characterization found that MePS is a branched-chain glucan with a weight-average molecular weight of 283.2 kDa. Additionally, MePS exhibited considerable antioxidant capacities in both in vitro biochemical assays and the H2O2-induced oxidative stress cell model. This work establishes a potential pathway for polysaccharide production, reducing reliance on plant and sugar-based feedstocks, while offering a new strategy for methane emission reduction.

AB - Bioconversion of methane into functional polysaccharides presents a promising strategy for mitigating methane-induced greenhouse gas emissions and addressing the limitations of plant-derived polysaccharide and sugar-based microbial polysaccharides production. In this study, the novel methane-derived polysaccharide (MePS) was obtained from a newly isolated methanotrophic bacterium Alkalicoccus glycogenes WONF2802. Structural characterization found that MePS is a branched-chain glucan with a weight-average molecular weight of 283.2 kDa. Additionally, MePS exhibited considerable antioxidant capacities in both in vitro biochemical assays and the H2O2-induced oxidative stress cell model. This work establishes a potential pathway for polysaccharide production, reducing reliance on plant and sugar-based feedstocks, while offering a new strategy for methane emission reduction.

KW - antioxidant

KW - methane

KW - methanotrophic bacterium

KW - polysaccharides

KW - structural characterization

UR - https://www.scopus.com/pages/publications/105003537637

U2 - 10.1021/acs.jafc.5c01087

DO - 10.1021/acs.jafc.5c01087

M3 - 文章

C2 - 40274597

AN - SCOPUS:105003537637

SN - 0021-8561

VL - 73

SP - 11019

EP - 11029

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 18

ER -

Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this