Measuring Redox Status of Melanoma Cells

Feng Liu-Smith; Tatiana B. Krasieva; Jing Liu; Jiankang Liu; Frank L. Meyskens

doi:10.1007/7651_2016_352

Measuring Redox Status of Melanoma Cells

Feng Liu-Smith
, Tatiana B. Krasieva
, Jing Liu
, Jiankang Liu
, Frank L. Meyskens

Health Science Center

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

Original language	English
Journal	Methods in Molecular Biology
Volume	2016
DOIs	https://doi.org/10.1007/7651_2016_352
State	Published - 2016

Keywords

Dichlorofluorescin diacetate (DCFDA)
Dihydroethidium (DHE)
Fluorometer
Hydrogen peroxide
Laser microscopy
Reactive oxygen species
Redox-sensitive GFP
Superoxide
ro1GFP
ro2GFP

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10.1007/7651_2016_352

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@article{dcd52634f33f47ad8eeda2025c5c01c9,

title = "Measuring Redox Status of Melanoma Cells",

abstract = "Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.",

keywords = "Dichlorofluorescin diacetate (DCFDA), Dihydroethidium (DHE), Fluorometer, Hydrogen peroxide, Laser microscopy, Reactive oxygen species, Redox-sensitive GFP, Superoxide, ro1GFP, ro2GFP",

author = "Feng Liu-Smith and Krasieva, \{Tatiana B.\} and Jing Liu and Jiankang Liu and Meyskens, \{Frank L.\}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",

year = "2016",

doi = "10.1007/7651\_2016\_352",

language = "英语",

volume = "2016",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - Measuring Redox Status of Melanoma Cells

AU - Liu-Smith, Feng

AU - Krasieva, Tatiana B.

AU - Liu, Jing

AU - Liu, Jiankang

AU - Meyskens, Frank L.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

AB - Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.

KW - Dichlorofluorescin diacetate (DCFDA)

KW - Dihydroethidium (DHE)

KW - Fluorometer

KW - Hydrogen peroxide

KW - Laser microscopy

KW - Reactive oxygen species

KW - Redox-sensitive GFP

KW - Superoxide

KW - ro1GFP

KW - ro2GFP

UR - https://www.scopus.com/pages/publications/85213681102

U2 - 10.1007/7651_2016_352

DO - 10.1007/7651_2016_352

M3 - 文章

AN - SCOPUS:85213681102

SN - 1064-3745

VL - 2016

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Measuring Redox Status of Melanoma Cells

Abstract

Keywords

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