Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices

A. Poghossian; T. S. Bronder; S. Scheja; C. Wu; T. Weinand; C. Metzger-Boddien; M. Keusgen; M. J. Schöning

doi:10.1016/j.proeng.2016.11.512

Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices

A. Poghossian
, T. S. Bronder
, S. Scheja
, C. Wu
, T. Weinand
, C. Metzger-Boddien
, M. Keusgen
, M. J. Schöning

Health Science Center

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.

Original language	English
Pages (from-to)	514-517
Number of pages	4
Journal	Procedia Engineering
Volume	168
DOIs	https://doi.org/10.1016/j.proeng.2016.11.512
State	Published - 2016
Event	30th Eurosensors Conference, Eurosensors 2016 - Budapest, Hungary Duration: 4 Sep 2016 → 7 Sep 2016

Keywords

DNA hybridization
field-effect sensor
label-free detection
polyelectrolyte
polymerase chain reaction (PCR)

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10.1016/j.proeng.2016.11.512

Cite this

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title = "Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices",

abstract = "A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.",

keywords = "DNA hybridization, field-effect sensor, label-free detection, polyelectrolyte, polymerase chain reaction (PCR)",

author = "A. Poghossian and Bronder, \{T. S.\} and S. Scheja and C. Wu and T. Weinand and C. Metzger-Boddien and M. Keusgen and Sch{\"o}ning, \{M. J.\}",

note = "Publisher Copyright: {\textcopyright} 2016 The Authors.; 30th Eurosensors Conference, Eurosensors 2016 ; Conference date: 04-09-2016 Through 07-09-2016",

year = "2016",

doi = "10.1016/j.proeng.2016.11.512",

language = "英语",

volume = "168",

pages = "514--517",

journal = "Procedia Engineering",

issn = "1877-7058",

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TY - JOUR

T1 - Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices

AU - Poghossian, A.

AU - Bronder, T. S.

AU - Scheja, S.

AU - Wu, C.

AU - Weinand, T.

AU - Metzger-Boddien, C.

AU - Keusgen, M.

AU - Schöning, M. J.

PY - 2016

Y1 - 2016

N2 - A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.

AB - A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.

KW - DNA hybridization

KW - field-effect sensor

KW - label-free detection

KW - polyelectrolyte

KW - polymerase chain reaction (PCR)

UR - https://www.scopus.com/pages/publications/85009949431

U2 - 10.1016/j.proeng.2016.11.512

DO - 10.1016/j.proeng.2016.11.512

M3 - 会议文章

AN - SCOPUS:85009949431

SN - 1877-7058

VL - 168

SP - 514

EP - 517

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 30th Eurosensors Conference, Eurosensors 2016

Y2 - 4 September 2016 through 7 September 2016

ER -

Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices

Abstract

Keywords

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