Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies

T. Bronder; C. S. Wu; A. Poghossian; C. F. Werner; M. Keusgend; M. J. Schöning

doi:10.1016/j.proeng.2014.11.647

Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies

T. Bronder
, C. S. Wu
, A. Poghossian
, C. F. Werner
, M. Keusgend
, M. J. Schöning

Health Science Center

Research output: Contribution to journal › Conference article › peer-review

15 Scopus citations

Abstract

Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO₂ and p-Si-SiO₂-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO₂ surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

Original language	English
Pages (from-to)	755-758
Number of pages	4
Journal	Procedia Engineering
Volume	87
DOIs	https://doi.org/10.1016/j.proeng.2014.11.647
State	Published - 2014
Event	28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy Duration: 7 Sep 2014 → 10 Sep 2014

Keywords

DNA hybridization
Field-effect sensor
Lable-free detection
LAPS

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10.1016/j.proeng.2014.11.647

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title = "Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies",

abstract = "Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).",

keywords = "DNA hybridization, Field-effect sensor, Lable-free detection, LAPS",

author = "T. Bronder and Wu, \{C. S.\} and A. Poghossian and Werner, \{C. F.\} and M. Keusgend and Sch{\"o}ning, \{M. J.\}",

note = "Publisher Copyright: {\textcopyright} 2014 The Authors. Published by Elsevier Ltd.; 28th European Conference on Solid-State Transducers, EUROSENSORS 2014 ; Conference date: 07-09-2014 Through 10-09-2014",

year = "2014",

doi = "10.1016/j.proeng.2014.11.647",

language = "英语",

volume = "87",

pages = "755--758",

journal = "Procedia Engineering",

issn = "1877-7058",

publisher = "Elsevier Ltd",

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TY - JOUR

T1 - Label-free detection of DNA hybridization with light-addressable potentiometric sensors

T2 - 28th European Conference on Solid-State Transducers, EUROSENSORS 2014

AU - Bronder, T.

AU - Wu, C. S.

AU - Poghossian, A.

AU - Werner, C. F.

AU - Keusgend, M.

AU - Schöning, M. J.

PY - 2014

Y1 - 2014

N2 - Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

AB - Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

KW - DNA hybridization

KW - Field-effect sensor

KW - Lable-free detection

KW - LAPS

UR - https://www.scopus.com/pages/publications/84923378555

U2 - 10.1016/j.proeng.2014.11.647

DO - 10.1016/j.proeng.2014.11.647

M3 - 会议文章

AN - SCOPUS:84923378555

SN - 1877-7058

VL - 87

SP - 755

EP - 758

JO - Procedia Engineering

JF - Procedia Engineering

Y2 - 7 September 2014 through 10 September 2014

ER -

Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies

Abstract

Keywords

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