Isothermal titration calorimetry in a miniaturized 3D-printed platform

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Abstract

Isothermal titration calorimetry can benefit from operating in miniaturized lab-on-a-chip (LOC) platforms since they enable quantitative, low-cost ITC measurements with improved response time, and reduced reagents consumption. In this paper, we present the first demonstration of a miniaturized 3D-printed LOC platform that integrates a polymeric MEMS-based measurement approach for quantitative ITC characterization of biomolecular interactions. The platform design features fully isolated cantilever-like calorimetric measurement structures that reduces thermal mass and improves measurement sensitivity. We demonstrate the utility of the platform with ITC measurements of titrating barium chloride with 18-crown-6 in a practically relevant concentration (0.2 mM) and a reusable manner.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages71-72
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 22 Oct 201726 Oct 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period22/10/1726/10/17

Keywords

  • 3D Printing
  • Isothermal Titration Calorimetry
  • Lab-on-a-chip
  • Thermoelectric Measurements

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