Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy

Chao Zhang; Xue Jun Sun; Dong Liu; Wei Liu; Jian Bao Zheng; Jun Kai Du; Xiao Feng Ling; Yuan Fu Zhang; Yi Zhuang Xu; Jin Guang Wu

doi:10.3964/j.issn.1000-0593(2013)06-1496-05

Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy

Chao Zhang
, Xue Jun Sun
, Dong Liu
, Wei Liu
, Jian Bao Zheng
, Jun Kai Du
, Xiao Feng Ling
, Yuan Fu Zhang
, Yi Zhuang Xu
, Jin Guang Wu

The First Affiliated Hospital of Xi’an Jiaotong University
Liaoning University of Traditional Chinese Medicine
Peking University

Research output: Contribution to journal › Article › peer-review

Abstract

The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR (Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2925, 1240 and 1085 cm^-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1400 cm^-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I₁₆₅₀/I₁₄₆₀, I₁₄₀₀/I₁₄₆₀ and I₁₂₄₀/I₁₄₆₀ increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I₁₇₄₀/I₁₄₆₀ decreased significantly (p<0.01). The relative intensity ratios of I₁₆₅₀/I₁₄₆₀, I₁₄₀₀/I₁₄₆₀ and I₁₂₄₀/I₁₄₆₀ also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.

Original language	English
Pages (from-to)	1496-1500
Number of pages	5
Journal	Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
Volume	33
Issue number	6
DOIs	https://doi.org/10.3964/j.issn.1000-0593(2013)06-1496-05
State	Published - Jun 2013
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell line
Cell nucleus
Fourier transform infrared spectra

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10.3964/j.issn.1000-0593(2013)06-1496-05

Cite this

@article{2c1f471c0025488f94866ae88623c574,

title = "Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy",

abstract = "The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR (Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2925, 1240 and 1085 cm-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1400 cm-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I1740/I1460 decreased significantly (p<0.01). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.",

keywords = "Cell line, Cell nucleus, Fourier transform infrared spectra",

author = "Chao Zhang and Sun, \{Xue Jun\} and Dong Liu and Wei Liu and Zheng, \{Jian Bao\} and Du, \{Jun Kai\} and Ling, \{Xiao Feng\} and Zhang, \{Yuan Fu\} and Xu, \{Yi Zhuang\} and Wu, \{Jin Guang\}",

year = "2013",

month = jun,

doi = "10.3964/j.issn.1000-0593(2013)06-1496-05",

language = "英语",

volume = "33",

pages = "1496--1500",

journal = "Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis",

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number = "6",

}

TY - JOUR

T1 - Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy

AU - Zhang, Chao

AU - Sun, Xue Jun

AU - Liu, Dong

AU - Liu, Wei

AU - Zheng, Jian Bao

AU - Du, Jun Kai

AU - Ling, Xiao Feng

AU - Zhang, Yuan Fu

AU - Xu, Yi Zhuang

AU - Wu, Jin Guang

PY - 2013/6

Y1 - 2013/6

N2 - The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR (Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2925, 1240 and 1085 cm-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1400 cm-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I1740/I1460 decreased significantly (p<0.01). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.

AB - The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR (Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2925, 1240 and 1085 cm-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1400 cm-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I1740/I1460 decreased significantly (p<0.01). The relative intensity ratios of I1650/I1460, I1400/I1460 and I1240/I1460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.

KW - Cell line

KW - Cell nucleus

KW - Fourier transform infrared spectra

UR - https://www.scopus.com/pages/publications/84879028541

U2 - 10.3964/j.issn.1000-0593(2013)06-1496-05

DO - 10.3964/j.issn.1000-0593(2013)06-1496-05

M3 - 文章

AN - SCOPUS:84879028541

SN - 1000-0593

VL - 33

SP - 1496

EP - 1500

JO - Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

JF - Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

IS - 6

ER -

Investigations on colorectum cancer cell lines and nucleus by FTIR spectroscopy

Abstract

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