Intestinal absorption and mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model

Min Yang; Guang Ji Wang; Yu Ping Xu; Dong Hui Pan; Xiao Lan Wu; Chen Rong Huang; Wen Cai Ye

Intestinal absorption and mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model

Min Yang
, Guang Ji Wang
, Yu Ping Xu
, Dong Hui Pan
, Xiao Lan Wu
, Chen Rong Huang
, Wen Cai Ye

Jiangsu Institute of Nuclear Medicine
China Pharmaceutical University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

OBJECTIVE: To investigate the intestinal absorption and the mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model. METHODS: The safety concentration of 23-hydroxybetulinic acid in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayer model was used to investigate the bi-directional transport of 23-hydroxybetulinic acid and the effect of time and drug concentration on the 23-hydroxybetulinic acid absorption. Drug concentration was measured by radioisotope tracing method and the apparent permeability coefficient (P_app) were calculated. RESULTS: In the Caco-2 cell model, the A→B P_app of 23-hydroxybetulinic acid was equal to the B→A P_app and the P_app kept almost constant with the selected concentration investigated. CONCLUSION: The 23-hydroxybetulinic acid absorption in Caco-2 cell model is a passive and transcellular transport. And the absorption of 23-hydroxybetulinic acid in the intestines is quite good.

Original language	English
Pages (from-to)	349-352
Number of pages	4
Journal	Chinese Pharmaceutical Journal
Volume	43
Issue number	5
State	Published - Mar 2008
Externally published	Yes

Keywords

23-hydroxybetulinic acid
Caco-2 cell model
Passive transport

Cite this

@article{15db27e4da384b628c48b35a859839d8,

title = "Intestinal absorption and mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model",

abstract = "OBJECTIVE: To investigate the intestinal absorption and the mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model. METHODS: The safety concentration of 23-hydroxybetulinic acid in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayer model was used to investigate the bi-directional transport of 23-hydroxybetulinic acid and the effect of time and drug concentration on the 23-hydroxybetulinic acid absorption. Drug concentration was measured by radioisotope tracing method and the apparent permeability coefficient (Papp) were calculated. RESULTS: In the Caco-2 cell model, the A→B Papp of 23-hydroxybetulinic acid was equal to the B→A Papp and the Papp kept almost constant with the selected concentration investigated. CONCLUSION: The 23-hydroxybetulinic acid absorption in Caco-2 cell model is a passive and transcellular transport. And the absorption of 23-hydroxybetulinic acid in the intestines is quite good.",

keywords = "23-hydroxybetulinic acid, Caco-2 cell model, Passive transport",

author = "Min Yang and Wang, \{Guang Ji\} and Xu, \{Yu Ping\} and Pan, \{Dong Hui\} and Wu, \{Xiao Lan\} and Huang, \{Chen Rong\} and Ye, \{Wen Cai\}",

year = "2008",

month = mar,

language = "英语",

volume = "43",

pages = "349--352",

journal = "Chinese Pharmaceutical Journal",

issn = "1001-2494",

publisher = "Chinese Pharmaceutical Association",

number = "5",

}

TY - JOUR

T1 - Intestinal absorption and mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model

AU - Yang, Min

AU - Wang, Guang Ji

AU - Xu, Yu Ping

AU - Pan, Dong Hui

AU - Wu, Xiao Lan

AU - Huang, Chen Rong

AU - Ye, Wen Cai

PY - 2008/3

Y1 - 2008/3

N2 - OBJECTIVE: To investigate the intestinal absorption and the mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model. METHODS: The safety concentration of 23-hydroxybetulinic acid in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayer model was used to investigate the bi-directional transport of 23-hydroxybetulinic acid and the effect of time and drug concentration on the 23-hydroxybetulinic acid absorption. Drug concentration was measured by radioisotope tracing method and the apparent permeability coefficient (Papp) were calculated. RESULTS: In the Caco-2 cell model, the A→B Papp of 23-hydroxybetulinic acid was equal to the B→A Papp and the Papp kept almost constant with the selected concentration investigated. CONCLUSION: The 23-hydroxybetulinic acid absorption in Caco-2 cell model is a passive and transcellular transport. And the absorption of 23-hydroxybetulinic acid in the intestines is quite good.

AB - OBJECTIVE: To investigate the intestinal absorption and the mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model. METHODS: The safety concentration of 23-hydroxybetulinic acid in Caco-2 cell was first selected by using MTT method. Then the Caco-2 cell monolayer model was used to investigate the bi-directional transport of 23-hydroxybetulinic acid and the effect of time and drug concentration on the 23-hydroxybetulinic acid absorption. Drug concentration was measured by radioisotope tracing method and the apparent permeability coefficient (Papp) were calculated. RESULTS: In the Caco-2 cell model, the A→B Papp of 23-hydroxybetulinic acid was equal to the B→A Papp and the Papp kept almost constant with the selected concentration investigated. CONCLUSION: The 23-hydroxybetulinic acid absorption in Caco-2 cell model is a passive and transcellular transport. And the absorption of 23-hydroxybetulinic acid in the intestines is quite good.

KW - 23-hydroxybetulinic acid

KW - Caco-2 cell model

KW - Passive transport

UR - https://www.scopus.com/pages/publications/53049107781

M3 - 文章

AN - SCOPUS:53049107781

SN - 1001-2494

VL - 43

SP - 349

EP - 352

JO - Chinese Pharmaceutical Journal

JF - Chinese Pharmaceutical Journal

IS - 5

ER -

Intestinal absorption and mechanism of 23-hydroxybetulinic acid using Caco-2 monolayer model

Abstract

Keywords

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