Interaction of sub-microsecond pulsed electric fiel wit bacteria cells

Priya R. Chalise; Stefano Perni; Gilbert Shama; Bucur M. Novac; Ivor R. Smith; Michael G. Kong

doi:10.1109/MODSYM.2006.365313

Interaction of sub-microsecond pulsed electric fiel wit bacteria cells

Priya R. Chalise
, Stefano Perni
, Gilbert Shama
, Bucur M. Novac
, Ivor R. Smith
, Michael G. Kong

School of Chemical Engineering and Technology

Loughborough University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

In the bacterial decontamination of liquid foods, the use of a sub microsecond pulsed electric field is of significant interest because it offers advantages such as negligible thermal effects, superior energy efficiency, and a much-reduced probability of medium breakdown. The reduced pulse duration also effects the mechanism by which sub microsecond pulses inactivate bacterial cells. We provide insight into possible bacterial inactivation mechanisms by sub-microsecond electric pulse with the aid of a common bacterium-wild type Escherichia coli and its mutants. Two pulsed power sources have been designed, developed and tested-one with a pulse duration of 32 ns, the other 700 ns, and both are capable of establishing sufficiently high electric field intensity. The experimental results suggest that bacterial inactivation by 700 ns pulses is due to electrical breakdown of the outer cell membrane, whereas inactivation by 32 ns pulses is possibly due to interaction with intracellular structures.

Original language	English
Title of host publication	2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop)
Pages	562-565
Number of pages	4
DOIs	https://doi.org/10.1109/MODSYM.2006.365313
State	Published - 2006
Event	2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop) - Washington, DC, United States Duration: 14 May 2006 → 18 May 2006

Publication series

Name	Conference Record of the International Power Modulator Symposium and High Voltage Workshop
ISSN (Print)	1076-8467

Conference

Conference	2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop)
Country/Territory	United States
City	Washington, DC
Period	14/05/06 → 18/05/06

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

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10.1109/MODSYM.2006.365313

Cite this

Chalise, P. R., Perni, S., Shama, G., Novac, B. M., Smith, I. R., & Kong, M. G. (2006). Interaction of sub-microsecond pulsed electric fiel wit bacteria cells. In 2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop) (pp. 562-565). Article 4216265 (Conference Record of the International Power Modulator Symposium and High Voltage Workshop). https://doi.org/10.1109/MODSYM.2006.365313

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title = "Interaction of sub-microsecond pulsed electric fiel wit bacteria cells",

abstract = "In the bacterial decontamination of liquid foods, the use of a sub microsecond pulsed electric field is of significant interest because it offers advantages such as negligible thermal effects, superior energy efficiency, and a much-reduced probability of medium breakdown. The reduced pulse duration also effects the mechanism by which sub microsecond pulses inactivate bacterial cells. We provide insight into possible bacterial inactivation mechanisms by sub-microsecond electric pulse with the aid of a common bacterium-wild type Escherichia coli and its mutants. Two pulsed power sources have been designed, developed and tested-one with a pulse duration of 32 ns, the other 700 ns, and both are capable of establishing sufficiently high electric field intensity. The experimental results suggest that bacterial inactivation by 700 ns pulses is due to electrical breakdown of the outer cell membrane, whereas inactivation by 32 ns pulses is possibly due to interaction with intracellular structures.",

author = "Chalise, \{Priya R.\} and Stefano Perni and Gilbert Shama and Novac, \{Bucur M.\} and Smith, \{Ivor R.\} and Kong, \{Michael G.\}",

year = "2006",

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pages = "562--565",

booktitle = "2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop)",

note = "2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop) ; Conference date: 14-05-2006 Through 18-05-2006",

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Chalise, PR, Perni, S, Shama, G, Novac, BM, Smith, IR & Kong, MG 2006, Interaction of sub-microsecond pulsed electric fiel wit bacteria cells. in 2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop)., 4216265, Conference Record of the International Power Modulator Symposium and High Voltage Workshop, pp. 562-565, 2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop), Washington, DC, United States, 14/05/06. https://doi.org/10.1109/MODSYM.2006.365313

Interaction of sub-microsecond pulsed electric fiel wit bacteria cells. / Chalise, Priya R.; Perni, Stefano; Shama, Gilbert et al.
2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop). 2006. p. 562-565 4216265 (Conference Record of the International Power Modulator Symposium and High Voltage Workshop).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Interaction of sub-microsecond pulsed electric fiel wit bacteria cells

AU - Chalise, Priya R.

AU - Perni, Stefano

AU - Shama, Gilbert

AU - Novac, Bucur M.

AU - Smith, Ivor R.

AU - Kong, Michael G.

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N2 - In the bacterial decontamination of liquid foods, the use of a sub microsecond pulsed electric field is of significant interest because it offers advantages such as negligible thermal effects, superior energy efficiency, and a much-reduced probability of medium breakdown. The reduced pulse duration also effects the mechanism by which sub microsecond pulses inactivate bacterial cells. We provide insight into possible bacterial inactivation mechanisms by sub-microsecond electric pulse with the aid of a common bacterium-wild type Escherichia coli and its mutants. Two pulsed power sources have been designed, developed and tested-one with a pulse duration of 32 ns, the other 700 ns, and both are capable of establishing sufficiently high electric field intensity. The experimental results suggest that bacterial inactivation by 700 ns pulses is due to electrical breakdown of the outer cell membrane, whereas inactivation by 32 ns pulses is possibly due to interaction with intracellular structures.

AB - In the bacterial decontamination of liquid foods, the use of a sub microsecond pulsed electric field is of significant interest because it offers advantages such as negligible thermal effects, superior energy efficiency, and a much-reduced probability of medium breakdown. The reduced pulse duration also effects the mechanism by which sub microsecond pulses inactivate bacterial cells. We provide insight into possible bacterial inactivation mechanisms by sub-microsecond electric pulse with the aid of a common bacterium-wild type Escherichia coli and its mutants. Two pulsed power sources have been designed, developed and tested-one with a pulse duration of 32 ns, the other 700 ns, and both are capable of establishing sufficiently high electric field intensity. The experimental results suggest that bacterial inactivation by 700 ns pulses is due to electrical breakdown of the outer cell membrane, whereas inactivation by 32 ns pulses is possibly due to interaction with intracellular structures.

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SN - 9781424400188

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Chalise PR, Perni S, Shama G, Novac BM, Smith IR, Kong MG. Interaction of sub-microsecond pulsed electric fiel wit bacteria cells. In 2006 IEEE International Power Modulator Conference, IPMC(27th Power Modulator Symposium and 2006 High Voltage Workshop). 2006. p. 562-565. 4216265. (Conference Record of the International Power Modulator Symposium and High Voltage Workshop). doi: 10.1109/MODSYM.2006.365313

Interaction of sub-microsecond pulsed electric fiel wit bacteria cells

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