Abstract
Objective: To explore the inhibitory effect of resveratrol (Res) on the expression of epidermal growth factor receptor (EGFR) in A431 cells and the action mechanisms. Methods: The effects of Res on A431 cell growth and the cell cycle were determined by MTT and flow cytometry, respectively. The effects of Res on EGFR kinase as well as EGFR, Akt and ERK mRNA expressions were determined by Lance assay and Real-time PCR. Its effects on protein level of EGFR, Akt and ERK expressions in A431 cells were determined by Western blot. Results: MTT assay showed that Res significantly inhibited the growth of A431 cells in a dose-dependent manner. The cell cycle was changed by Res. The results of Lance and RT-PCR showed that Res did not inhibit the activity of EGFR kinase, EGFR, Akt or ERK mRNA expressions obviously. The results of Western blot showed that Res down-regulated the expressions of EGFR and ERK obviously (P<0.05). Conclusion: Res can inhibit the proliferation of A431 cells and change cell cycle, which is probably related to the down-regulation of EGFR and ERK expressions.
| Original language | English |
|---|---|
| Pages (from-to) | 185-188 |
| Number of pages | 4 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 34 |
| Issue number | 2 |
| State | Published - Mar 2013 |
Keywords
- A431cell
- Epithelial growth factor receptor (EGFR)
- Resveratrol
- Tumor inhibition