In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Sheng Li; Guoan Zhang; Yanping Liu; Qinru Sun; Lu Yang; Junli Dong; Bowen Zhang; Su Chen

doi:10.1016/j.xpro.2021.100601

In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Sheng Li
, Guoan Zhang
, Yanping Liu
, Qinru Sun
, Lu Yang
, Junli Dong
, Bowen Zhang
, Su Chen

Health Science Center

Jining Medical College
Xi'an Jiaotong University
Henan University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

Original language	English
Article number	100601
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100601
State	Published - 18 Jun 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Cell separation/fractionation
Molecular Biology
Protein expression and purification

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10.1016/j.xpro.2021.100601

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title = "In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions",

abstract = "Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).",

keywords = "Cancer, Cell separation/fractionation, Molecular Biology, Protein expression and purification",

author = "Sheng Li and Guoan Zhang and Yanping Liu and Qinru Sun and Lu Yang and Junli Dong and Bowen Zhang and Su Chen",

note = "Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jun,

day = "18",

doi = "10.1016/j.xpro.2021.100601",

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TY - JOUR

T1 - In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

AU - Li, Sheng

AU - Zhang, Guoan

AU - Liu, Yanping

AU - Sun, Qinru

AU - Yang, Lu

AU - Dong, Junli

AU - Zhang, Bowen

AU - Chen, Su

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

AB - Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

KW - Cancer

KW - Cell separation/fractionation

KW - Molecular Biology

KW - Protein expression and purification

UR - https://www.scopus.com/pages/publications/85107822255

U2 - 10.1016/j.xpro.2021.100601

DO - 10.1016/j.xpro.2021.100601

M3 - 文章

C2 - 34169294

AN - SCOPUS:85107822255

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 100601

ER -

In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Abstract

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Keywords

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