In-vitro evaluation of accuracy of dynamic contrast-enhanced ultrasound (DCEUS)-based parametric perfusion imaging with respiratory motion-compensation

Purpose: The accuracy of multi-parametric perfusion imaging (PPI) based on dynamic contrast-enhanced ultrasound is disturbed by the respiratory motion in some cases, especially during characterizing hemodynamic features of abdominal tumor angiogenesis. This study aimed to effectively remove those disturbances on PPI and evaluate its accuracy. Method: The respiratory motion-compensation (rMoCo) strategy in PPI was modified by employing non-negative matrix factorization combined with phase-by-phase compensation. According to the known and controllable ground truths in in-vitro perfusion experiments, the accuracy of the modified rMoCo strategy was further evaluated from multiple perspectives in a simulated dual-vessel flow phantom. Results: Compared with that of PPIs without rMoCo, the mean correlation coefficient between six PPIs with rMoCo and the corresponding static PPIs was up to 0.98 ± 0.01 and improved by 0.17 ± 0.04 (P < 0.05). The estimated error of vascular diameter decreased from 87.85% (P < 0.05) to 7.25% (P < 0.05) after rMoCo. PPIs with rMoCo were significantly consistent with static PPIs without respiratory motion disturbances. Conclusions: These quantitative results illustrated the disturbances induced by respiratory motion were effectively removed and the accuracy of PPIs was significantly improved. The partial parabolic and bimodal hemodynamic characteristics and the anatomical structures and sizes were accurately quantified and depicted by PPIs with rMoCo. The modified method can benefit physicians in providing accurate diagnoses and in developing appropriate therapeutic strategies for abdominal diseases.