Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

De Wen Zhang; Fan Wu; Jian Wang; Michael Watkinson; Steffi Krause

doi:10.1016/j.elecom.2016.08.017

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

De Wen Zhang
, Fan Wu
, Jian Wang
, Michael Watkinson
, Steffi Krause

Queen Mary University of London
China Academy of Engineering Physics

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

A light-addressable potentiometric sensor (LAPS) was developed for the image detection of multilayer yeast Saccharomyces cerevisiae. By modifying silicon-on-sapphire (SOS) with a monolayer of 1,8-nonadiyne, an inert LAPS substrate, insensitive to pH was obtained. Low gelling temperature agarose gel was shown to be an effective immobilization reagent for yeast cells in LAPS measurements without causing a background signal. The photocurrent images obtained were shown to be induced by the surface negative charge and the local impedance of the yeast cells.

Original language	English
Pages (from-to)	41-45
Number of pages	5
Journal	Electrochemistry Communications
Volume	72
DOIs	https://doi.org/10.1016/j.elecom.2016.08.017
State	Published - 1 Nov 2016
Externally published	Yes

Keywords

Cell imaging
LAPS
Saccharomyces cerevisiae
Scanning Photo-induced Impedance Microscopy (SPIM)

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10.1016/j.elecom.2016.08.017

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title = "Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)",

abstract = "A light-addressable potentiometric sensor (LAPS) was developed for the image detection of multilayer yeast Saccharomyces cerevisiae. By modifying silicon-on-sapphire (SOS) with a monolayer of 1,8-nonadiyne, an inert LAPS substrate, insensitive to pH was obtained. Low gelling temperature agarose gel was shown to be an effective immobilization reagent for yeast cells in LAPS measurements without causing a background signal. The photocurrent images obtained were shown to be induced by the surface negative charge and the local impedance of the yeast cells.",

keywords = "Cell imaging, LAPS, Saccharomyces cerevisiae, Scanning Photo-induced Impedance Microscopy (SPIM)",

author = "Zhang, \{De Wen\} and Fan Wu and Jian Wang and Michael Watkinson and Steffi Krause",

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year = "2016",

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doi = "10.1016/j.elecom.2016.08.017",

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T1 - Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

AU - Zhang, De Wen

AU - Wu, Fan

AU - Wang, Jian

AU - Watkinson, Michael

AU - Krause, Steffi

PY - 2016/11/1

Y1 - 2016/11/1

N2 - A light-addressable potentiometric sensor (LAPS) was developed for the image detection of multilayer yeast Saccharomyces cerevisiae. By modifying silicon-on-sapphire (SOS) with a monolayer of 1,8-nonadiyne, an inert LAPS substrate, insensitive to pH was obtained. Low gelling temperature agarose gel was shown to be an effective immobilization reagent for yeast cells in LAPS measurements without causing a background signal. The photocurrent images obtained were shown to be induced by the surface negative charge and the local impedance of the yeast cells.

AB - A light-addressable potentiometric sensor (LAPS) was developed for the image detection of multilayer yeast Saccharomyces cerevisiae. By modifying silicon-on-sapphire (SOS) with a monolayer of 1,8-nonadiyne, an inert LAPS substrate, insensitive to pH was obtained. Low gelling temperature agarose gel was shown to be an effective immobilization reagent for yeast cells in LAPS measurements without causing a background signal. The photocurrent images obtained were shown to be induced by the surface negative charge and the local impedance of the yeast cells.

KW - Cell imaging

KW - LAPS

KW - Saccharomyces cerevisiae

KW - Scanning Photo-induced Impedance Microscopy (SPIM)

UR - https://www.scopus.com/pages/publications/84985945340

U2 - 10.1016/j.elecom.2016.08.017

DO - 10.1016/j.elecom.2016.08.017

M3 - 文章

AN - SCOPUS:84985945340

SN - 1388-2481

VL - 72

SP - 41

EP - 45

JO - Electrochemistry Communications

JF - Electrochemistry Communications

ER -

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

Abstract

Keywords

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