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Identification of TGF-beta/Smads pathway in human prostate cancer cell lines and its significance

  • Kai jie Wu
  • , Guang feng Zhu
  • , Dong Zhang
  • , Jin Zeng
  • , Xin yang Wang
  • , Yan Xue
  • , Lin lin Zhang
  • , Da lin He
  • Xi'an Jiaotong University

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

OBJECTIVE: To determine the TGF-beta/Smads signal pathway in different human prostate cancer cell lines, and to explore the role of the TGF-beta/Smads pathway in the progression and metastasis of prostate cancer and its possible mechanisms. METHODS: We detected the expressions of the key proteins involved in the TGF-beta/Smads pathway, TbetaR II, Smad2/3, p-Smad2 and Smad4, in prostate cancer cell lines LNCaP, PC-3, DU145, IF11 and IA8 with different metastatic potentials by Western blotting. RESULTS: TbetaR II was expressed highly in PC-3, DU145, IF11 and IA8, but extremely lowly in LNCaP. Smad2/3 was highly expressed in all the cell lines with different intensity, while p-Smad2 only in PC-3 and DU145. The expression of Smad4 was detected in LNCaP, PC-3 and DU145, but not in IF11 and IA8. CONCLUSION: The status of the TGF-beta/Smads pathway differs in the cell lines with different metastatic potentials, only active in PC-3 and DU145. The TGF-beta/Smads pathway may be involved in the invasion and metastasis of prostate cancer through altering the expressions of the key proteins in it.

Original languageEnglish
Pages (from-to)920-924
Number of pages5
JournalZhonghua nan ke xue = National journal of andrology
Volume15
Issue number10
StatePublished - Oct 2009

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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