Human CD3⁺CD56⁺NKT-like cells express a range of complement receptors and C3 activation has negative effects on these cell activity and effector function

CD3⁺CD56⁺NKT-like cells are a rare population of lymphocytes that serve important roles in various types of immune-related diseases, and particularly in cancer. The complement system regulates inflammatory and immune responses by interacting with complement receptors expressed on a range of immune cells. However, whether CD3⁺CD56⁺NKT-like cells are regulated by the complement system has still not been definitively determined. In the present study, the expression of complement receptors and regulators in gated CD3⁺CD56⁺NKT-like cells isolated from human peripheral blood was assessed using PCR and flow cytometry. The results showed that human CD3⁺CD56⁺NKT-like cells expressed a range of complement receptors and regulators, such as CR3, C3aR, C5aR, C5L2, CD46 and CD55. Furthermore, the presence of complement component 3 (C3), a key component in complement activation in culture supernatant, mitigated the activity, IFN-γ production and killing function of CD3⁺CD56⁺NKT-like cells. The present study provides evidences supporting the relationship between complement activation and functional modulation of CD3⁺CD56⁺NKT-like cells, expanding our knowledge of the complement regulatory network, and also highlighting a potential target for treatment of numerous immune-related diseases, particularly NKT cell-based tumor adoptive immunotherapy.