HPLC-fingerprint analysis of the active part of Radix Ligusticum Chuangxiong Hort

Objective: To establish a HPLC-fingerprint pattern (HPLC-FPS) for the active part of Radix Ligusticum Chuangxiong Hort. and make a comparative study of HPLC-FPS of seven kinds of Radix Ligusticum Chuangxiong Hort. from different sources. Methods: The samples were extracted with ethanol by suPersonic technique for 20 min, and extracted with ethyl acetate. The ODS column was used as analytical column, the mobile phase consisted of methanol-water-acetic acid (30:70:0.5), and the flow rate was 1 mL·min^-1 with UV detection at 276 nm. Results: The method was proven to be plecise with good reproducibility and the RSD was 1. 68% and 1. 04% using ferulic acid as the standard component, respectively. The spectrum of the chromatograms was similar, but the ratio of common peaks' area of different samples was different. Conclusion: This method can be used to differentiate conveniently and rapidly Radix Ligusticum Chuangxiong Hort. from different sources.