High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies

S. Saouros; T. M.A. Blumenschein; K. Sawmynaden; J. Marchant; T. Koutroukides; B. Liu; P. Simpson; E. P. Carpenter; S. J. Matthews

doi:10.2174/092986607780782876

High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies

S. Saouros
, T. M.A. Blumenschein
, K. Sawmynaden
, J. Marchant
, T. Koutroukides
, B. Liu
, P. Simpson
, E. P. Carpenter
, S. J. Matthews

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.

Original language	English
Pages (from-to)	411-415
Number of pages	5
Journal	Protein and Peptide Letters
Volume	14
Issue number	5
DOIs	https://doi.org/10.2174/092986607780782876
State	Published - May 2007
Externally published	Yes

Keywords

Cysteine-rich proteins
Micronemal proteins
NMR
Toxoplasma gondii
X-ray crystallography

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10.2174/092986607780782876

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title = "High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies",

abstract = "The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.",

keywords = "Cysteine-rich proteins, Micronemal proteins, NMR, Toxoplasma gondii, X-ray crystallography",

author = "S. Saouros and Blumenschein, \{T. M.A.\} and K. Sawmynaden and J. Marchant and T. Koutroukides and B. Liu and P. Simpson and Carpenter, \{E. P.\} and Matthews, \{S. J.\}",

year = "2007",

month = may,

doi = "10.2174/092986607780782876",

language = "英语",

volume = "14",

pages = "411--415",

journal = "Protein and Peptide Letters",

issn = "0929-8665",

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TY - JOUR

T1 - High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies

AU - Saouros, S.

AU - Blumenschein, T. M.A.

AU - Sawmynaden, K.

AU - Marchant, J.

AU - Koutroukides, T.

AU - Liu, B.

AU - Simpson, P.

AU - Carpenter, E. P.

AU - Matthews, S. J.

PY - 2007/5

Y1 - 2007/5

N2 - The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.

AB - The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application.

KW - Cysteine-rich proteins

KW - Micronemal proteins

KW - NMR

KW - Toxoplasma gondii

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/34249665632

U2 - 10.2174/092986607780782876

DO - 10.2174/092986607780782876

M3 - 文章

C2 - 17584164

AN - SCOPUS:34249665632

SN - 0929-8665

VL - 14

SP - 411

EP - 415

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 5

ER -

High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies

Abstract

Keywords

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