High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

Wenming Zheng; Lili Huang; Jinqun Huang; Xiaojie Wang; Xianming Chen; Jie Zhao; Jun Guo; Hua Zhuang; Chuangzhao Qiu; Jie Liu; Huiquan Liu; Xueling Huang; Guoliang Pei; Gangming Zhan; Chunlei Tang; Yulin Cheng; Minjie Liu; Jinshan Zhang; Zhongtao Zhao; Shijie Zhang; Qingmei Han; Dejun Han; Hongchang Zhang; Jing Zhao; Xiaoning Gao; Jianfeng Wang; Peixiang Ni; Wei Dong; Linfeng Yang; Huanming Yang; Jin Rong Xu; Gengyun Zhang; Zhensheng Kang

doi:10.1038/ncomms3673

High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

Wenming Zheng
, Lili Huang
, Jinqun Huang
, Xiaojie Wang
, Xianming Chen
, Jie Zhao
, Jun Guo
, Hua Zhuang
, Chuangzhao Qiu
, Jie Liu
, Huiquan Liu
, Xueling Huang
, Guoliang Pei
, Gangming Zhan
, Chunlei Tang
, Yulin Cheng
, Minjie Liu
, Jinshan Zhang
, Zhongtao Zhao
, Shijie Zhang

Qingmei Han, Dejun Han, Hongchang Zhang, Jing Zhao, Xiaoning Gao, Jianfeng Wang, Peixiang Ni, Wei Dong, Linfeng Yang, Huanming Yang, Jin Rong Xu, Gengyun Zhang, Zhensheng Kang

Research output: Contribution to journal › Article › peer-review

206 Scopus citations

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

Original language	English
Article number	2673
Journal	Nature Communications
Volume	4
DOIs	https://doi.org/10.1038/ncomms3673
State	Published - 23 Oct 2013
Externally published	Yes

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Zheng, W., Huang, L., Huang, J., Wang, X., Chen, X., Zhao, J., Guo, J., Zhuang, H., Qiu, C., Liu, J., Liu, H., Huang, X., Pei, G., Zhan, G., Tang, C., Cheng, Y., Liu, M., Zhang, J., Zhao, Z., ... Kang, Z. (2013). High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus. Nature Communications, 4, Article 2673. https://doi.org/10.1038/ncomms3673

@article{a8f5b5384c8d4f2c87ddf1a9e705ebf3,

title = "High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus",

abstract = "Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35\% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.",

author = "Wenming Zheng and Lili Huang and Jinqun Huang and Xiaojie Wang and Xianming Chen and Jie Zhao and Jun Guo and Hua Zhuang and Chuangzhao Qiu and Jie Liu and Huiquan Liu and Xueling Huang and Guoliang Pei and Gangming Zhan and Chunlei Tang and Yulin Cheng and Minjie Liu and Jinshan Zhang and Zhongtao Zhao and Shijie Zhang and Qingmei Han and Dejun Han and Hongchang Zhang and Jing Zhao and Xiaoning Gao and Jianfeng Wang and Peixiang Ni and Wei Dong and Linfeng Yang and Huanming Yang and Xu, \{Jin Rong\} and Gengyun Zhang and Zhensheng Kang",

year = "2013",

month = oct,

day = "23",

doi = "10.1038/ncomms3673",

language = "英语",

volume = "4",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

}

Zheng, W, Huang, L, Huang, J, Wang, X, Chen, X, Zhao, J, Guo, J, Zhuang, H, Qiu, C, Liu, J, Liu, H, Huang, X, Pei, G, Zhan, G, Tang, C, Cheng, Y, Liu, M, Zhang, J, Zhao, Z, Zhang, S, Han, Q, Han, D, Zhang, H, Zhao, J, Gao, X, Wang, J, Ni, P, Dong, W, Yang, L, Yang, H, Xu, JR, Zhang, G & Kang, Z 2013, 'High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus', Nature Communications, vol. 4, 2673. https://doi.org/10.1038/ncomms3673

TY - JOUR

T1 - High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

AU - Zheng, Wenming

AU - Huang, Lili

AU - Huang, Jinqun

AU - Wang, Xiaojie

AU - Chen, Xianming

AU - Zhao, Jie

AU - Guo, Jun

AU - Zhuang, Hua

AU - Qiu, Chuangzhao

AU - Liu, Jie

AU - Liu, Huiquan

AU - Huang, Xueling

AU - Pei, Guoliang

AU - Zhan, Gangming

AU - Tang, Chunlei

AU - Cheng, Yulin

AU - Liu, Minjie

AU - Zhang, Jinshan

AU - Zhao, Zhongtao

AU - Zhang, Shijie

AU - Han, Qingmei

AU - Han, Dejun

AU - Zhang, Hongchang

AU - Zhao, Jing

AU - Gao, Xiaoning

AU - Wang, Jianfeng

AU - Ni, Peixiang

AU - Dong, Wei

AU - Yang, Linfeng

AU - Yang, Huanming

AU - Xu, Jin Rong

AU - Zhang, Gengyun

AU - Kang, Zhensheng

PY - 2013/10/23

Y1 - 2013/10/23

N2 - Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

AB - Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat. Here we report a 110-Mb draft sequence of Pst isolate CY32, obtained using a 'fosmid-to-fosmid' strategy, to better understand its race evolution and pathogenesis. The Pst genome is highly heterozygous and contains 25,288 protein-coding genes. Compared with non-obligate fungal pathogens, Pst has a more diverse gene composition and more genes encoding secreted proteins. Re-sequencing analysis indicates significant genetic variation among six isolates collected from different continents. Approximately 35% of SNPs are in the coding sequence regions, and half of them are non-synonymous. High genetic diversity in Pst suggests that sexual reproduction has an important role in the origin of different regional races. Our results show the effectiveness of the 'fosmid-to-fosmid' strategy for sequencing dikaryotic genomes and the feasibility of genome analysis to understand race evolution in Pst and other obligate pathogens.

UR - https://www.scopus.com/pages/publications/84889261375

U2 - 10.1038/ncomms3673

DO - 10.1038/ncomms3673

M3 - 文章

C2 - 24150273

AN - SCOPUS:84889261375

SN - 2041-1723

VL - 4

JO - Nature Communications

JF - Nature Communications

M1 - 2673

ER -

High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus

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