TY - JOUR
T1 - Hepatitis b virus expression and replication in ovum and the influencing factors
AU - Kong, Ying
AU - Ye, Feng
AU - Jin, Yan
AU - Shi, Juanzi
AU - Qiu, Hongtao
AU - Lin, Shumei
N1 - Publisher Copyright:
© 2016 Saudi Journal of Gastroenterology (Official journal of The Saudi Gastroenterology Association) Published by Wolters Kluwer - Medknow.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Background/Aim: The aim of this study was to investigate the factors that influence hepatitis B virus (HBV) expression and replication in the ovum.Materials and Methods: Immunohistochemistry and in situ hybridization techniques were used to assay the distributions of HBcAg, HBV DNA, and HBV mRNA in ovarian tissues and the ovum in 50 patients with chronic HBV infection. HBeAg and HBV DNA in the serum were also detected. Comparisons of categorical data were performed using McNemar test. Results: The positive rates of HBcAg, HBV DNA, and HBV mRNA in ovum and ovarian tissues of high replication group were significantly higher than low replication group (χ2 = 15.04, P< 0.05; χ2 = 12.96, P<0.05; χ2 = 19.36, P< 0.05; respectively). High positive rates of HBcAg, HBV DNA, and HBV mRNA in ovum and ovarian tissues were found in women with HBeAg-positive than HBeAg-negative (χ2 = 113.14,P< 0.05; χ2 = 11.13, P< 0.05; χ2 = 17.39, P< 0.05; respectively). Conclusion: HBV can infect and replicate in the ovary and ovum. Maternal HBeAg status and HBV DNA levels are important influencing factors.
AB - Background/Aim: The aim of this study was to investigate the factors that influence hepatitis B virus (HBV) expression and replication in the ovum.Materials and Methods: Immunohistochemistry and in situ hybridization techniques were used to assay the distributions of HBcAg, HBV DNA, and HBV mRNA in ovarian tissues and the ovum in 50 patients with chronic HBV infection. HBeAg and HBV DNA in the serum were also detected. Comparisons of categorical data were performed using McNemar test. Results: The positive rates of HBcAg, HBV DNA, and HBV mRNA in ovum and ovarian tissues of high replication group were significantly higher than low replication group (χ2 = 15.04, P< 0.05; χ2 = 12.96, P<0.05; χ2 = 19.36, P< 0.05; respectively). High positive rates of HBcAg, HBV DNA, and HBV mRNA in ovum and ovarian tissues were found in women with HBeAg-positive than HBeAg-negative (χ2 = 113.14,P< 0.05; χ2 = 11.13, P< 0.05; χ2 = 17.39, P< 0.05; respectively). Conclusion: HBV can infect and replicate in the ovary and ovum. Maternal HBeAg status and HBV DNA levels are important influencing factors.
KW - Expression
KW - hepatitis B virus
KW - influencing factors
KW - ovum
KW - replication
UR - https://www.scopus.com/pages/publications/84969922059
U2 - 10.4103/1319-3767.182456
DO - 10.4103/1319-3767.182456
M3 - 文章
C2 - 27184640
AN - SCOPUS:84969922059
SN - 1319-3767
VL - 22
SP - 215
EP - 219
JO - Saudi Journal of Gastroenterology
JF - Saudi Journal of Gastroenterology
IS - 3
ER -
