Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile

Jing Jing; Weichao Ren; Xijing Chen; Yue Wang; Qiaoling Yu; Guangji Wang; Andrew K. Davey; Jiping Wang; Ning Jing

doi:10.2133/dmpk.23.175

Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile

Jing Jing
, Weichao Ren
, Xijing Chen
, Yue Wang
, Qiaoling Yu
, Guangji Wang
, Andrew K. Davey
, Jiping Wang
, Ning Jing

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LCMS total ion chromatogram (TIC). Taken together with the susceptible nature to β-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Drug Metabolism and Pharmacokinetics
Volume	23
Issue number	3
DOIs	https://doi.org/10.2133/dmpk.23.175
State	Published - 2008
Externally published	Yes

Keywords

Bile
Diconjugate
Glycyrrhetic acid
LC-MS
Metabolites

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10.2133/dmpk.23.175

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title = "Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile",

abstract = "To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LCMS total ion chromatogram (TIC). Taken together with the susceptible nature to β-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.",

keywords = "Bile, Diconjugate, Glycyrrhetic acid, LC-MS, Metabolites",

author = "Jing Jing and Weichao Ren and Xijing Chen and Yue Wang and Qiaoling Yu and Guangji Wang and Davey, \{Andrew K.\} and Jiping Wang and Ning Jing",

year = "2008",

doi = "10.2133/dmpk.23.175",

language = "英语",

volume = "23",

pages = "175--180",

journal = "Drug Metabolism and Pharmacokinetics",

issn = "1347-4367",

publisher = "Japanese Society for the Study of Xenobiotics",

number = "3",

}

TY - JOUR

T1 - Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile

AU - Jing, Jing

AU - Ren, Weichao

AU - Chen, Xijing

AU - Wang, Yue

AU - Yu, Qiaoling

AU - Wang, Guangji

AU - Davey, Andrew K.

AU - Wang, Jiping

AU - Jing, Ning

PY - 2008

Y1 - 2008

N2 - To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LCMS total ion chromatogram (TIC). Taken together with the susceptible nature to β-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.

AB - To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LCMS total ion chromatogram (TIC). Taken together with the susceptible nature to β-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.

KW - Bile

KW - Diconjugate

KW - Glycyrrhetic acid

KW - LC-MS

KW - Metabolites

UR - https://www.scopus.com/pages/publications/48849092904

U2 - 10.2133/dmpk.23.175

DO - 10.2133/dmpk.23.175

M3 - 文章

C2 - 18574321

AN - SCOPUS:48849092904

SN - 1347-4367

VL - 23

SP - 175

EP - 180

JO - Drug Metabolism and Pharmacokinetics

JF - Drug Metabolism and Pharmacokinetics

IS - 3

ER -

Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile

Abstract

Keywords

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