GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

Wei Wang; Ruiying Sun; Lizhong Zeng; Yang Chen; Na Zhang; Shiguang Cao; Shanshan Deng; Xia Meng; Shuanying Yang

doi:10.1016/j.lfs.2021.119439

GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

Wei Wang
, Ruiying Sun
, Lizhong Zeng
, Yang Chen
, Na Zhang
, Shiguang Cao
, Shanshan Deng
, Xia Meng
, Shuanying Yang

The Second Affiliated Hospital of Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Aims: Our study aimed to investigate the function of GALNT2 in lung adenocarcinoma (LUAD). Main methods: We used network tools and tissue microarray immunohistochemistry to measure the expression levels of GALNT2 in LUAD. Kaplan-Meier curves and Cox regression methods were used in survival analysis. We detected the role of GALNT2 in cell lines by Cell Counting Kit-8, colony formation, transwell, and wound healing assays. We performed Western blotting to evaluate downstream protein levels. Key findings: GALNT2 was highly expressed in LUAD samples and indicated a poor prognosis. Knockdown of GALNT2 suppressed cell line proliferation, migration, and invasion abilities, while overexpression of GALNT2 enhanced those phenotypes. Moreover, GALNT2 activated Notch/Hes1-PTEN-PI3K/Akt signaling axis. Significance: Our data confirmed the cancer-promoting effect of GALNT2, and might provide a new approach for LUAD therapy.

Original language	English
Article number	119439
Journal	Life Sciences
Volume	276
DOIs	https://doi.org/10.1016/j.lfs.2021.119439
State	Published - 1 Jul 2021
Externally published	Yes

Keywords

GALNT2
Lung adenocarcinoma
Metastasis
Notch
Proliferation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.lfs.2021.119439

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@article{d21435099ca24030aaef3008796600be,

title = "GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma",

abstract = "Aims: Our study aimed to investigate the function of GALNT2 in lung adenocarcinoma (LUAD). Main methods: We used network tools and tissue microarray immunohistochemistry to measure the expression levels of GALNT2 in LUAD. Kaplan-Meier curves and Cox regression methods were used in survival analysis. We detected the role of GALNT2 in cell lines by Cell Counting Kit-8, colony formation, transwell, and wound healing assays. We performed Western blotting to evaluate downstream protein levels. Key findings: GALNT2 was highly expressed in LUAD samples and indicated a poor prognosis. Knockdown of GALNT2 suppressed cell line proliferation, migration, and invasion abilities, while overexpression of GALNT2 enhanced those phenotypes. Moreover, GALNT2 activated Notch/Hes1-PTEN-PI3K/Akt signaling axis. Significance: Our data confirmed the cancer-promoting effect of GALNT2, and might provide a new approach for LUAD therapy.",

keywords = "GALNT2, Lung adenocarcinoma, Metastasis, Notch, Proliferation",

author = "Wei Wang and Ruiying Sun and Lizhong Zeng and Yang Chen and Na Zhang and Shiguang Cao and Shanshan Deng and Xia Meng and Shuanying Yang",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2021",

month = jul,

day = "1",

doi = "10.1016/j.lfs.2021.119439",

language = "英语",

volume = "276",

journal = "Life Sciences",

issn = "0024-3205",

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}

TY - JOUR

T1 - GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

AU - Wang, Wei

AU - Sun, Ruiying

AU - Zeng, Lizhong

AU - Chen, Yang

AU - Zhang, Na

AU - Cao, Shiguang

AU - Deng, Shanshan

AU - Meng, Xia

AU - Yang, Shuanying

PY - 2021/7/1

Y1 - 2021/7/1

N2 - Aims: Our study aimed to investigate the function of GALNT2 in lung adenocarcinoma (LUAD). Main methods: We used network tools and tissue microarray immunohistochemistry to measure the expression levels of GALNT2 in LUAD. Kaplan-Meier curves and Cox regression methods were used in survival analysis. We detected the role of GALNT2 in cell lines by Cell Counting Kit-8, colony formation, transwell, and wound healing assays. We performed Western blotting to evaluate downstream protein levels. Key findings: GALNT2 was highly expressed in LUAD samples and indicated a poor prognosis. Knockdown of GALNT2 suppressed cell line proliferation, migration, and invasion abilities, while overexpression of GALNT2 enhanced those phenotypes. Moreover, GALNT2 activated Notch/Hes1-PTEN-PI3K/Akt signaling axis. Significance: Our data confirmed the cancer-promoting effect of GALNT2, and might provide a new approach for LUAD therapy.

AB - Aims: Our study aimed to investigate the function of GALNT2 in lung adenocarcinoma (LUAD). Main methods: We used network tools and tissue microarray immunohistochemistry to measure the expression levels of GALNT2 in LUAD. Kaplan-Meier curves and Cox regression methods were used in survival analysis. We detected the role of GALNT2 in cell lines by Cell Counting Kit-8, colony formation, transwell, and wound healing assays. We performed Western blotting to evaluate downstream protein levels. Key findings: GALNT2 was highly expressed in LUAD samples and indicated a poor prognosis. Knockdown of GALNT2 suppressed cell line proliferation, migration, and invasion abilities, while overexpression of GALNT2 enhanced those phenotypes. Moreover, GALNT2 activated Notch/Hes1-PTEN-PI3K/Akt signaling axis. Significance: Our data confirmed the cancer-promoting effect of GALNT2, and might provide a new approach for LUAD therapy.

KW - GALNT2

KW - Lung adenocarcinoma

KW - Metastasis

KW - Notch

KW - Proliferation

UR - https://www.scopus.com/pages/publications/85105762832

U2 - 10.1016/j.lfs.2021.119439

DO - 10.1016/j.lfs.2021.119439

M3 - 文章

C2 - 33785338

AN - SCOPUS:85105762832

SN - 0024-3205

VL - 276

JO - Life Sciences

JF - Life Sciences

M1 - 119439

ER -

GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma

Abstract

Keywords

UN SDGs

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