Formation characteristics of bacteria and fungi in sewers: In terms of signal molecule generation

Bio-metabolism of diverse communities is the main reason of water quality variation in sewers, and the signal molecule generation of communities is dementated to be the key regulation procedure for community metabolism. To reveal the mechanism of pollutant biotransformation in complex sewer environment, this study explored the formation of bacteria and fungi and the signal molecule transduction characteristics in a pilot sewer. In this study, several kinds of signal molecules that produced by bacteria and fungi (C4-HSL, C6-HSL, C8-HSL, farnesol and tyrosol) were detected along the formation process of sewer biofilms. The results showed that, in the early stage, bacterial AHLs signaling molecules are beneficial to the synthesis of EPS, providing a good material basis for the growth of bacterial flora. In addition, tyrosol stimulates the formation of embryonic tubes in yeast cells, further promoting the growth of hyphae. At the later stage, AHLs signaling molecules and tyrosol jointly promoted the growth of biofilms. In conclusion, it is precisely because of the coexistence of bacteria and fungi in the sewer system that the generated signal molecules can jointly promote the synthesis and growth of biofilms through different pathways, and have positive feedback on the biodegradation of various pollutants. Based on the exploration, the ecological patterns of bacterial-fungal communities in urban sewer system were proposed and it could improve the understanding on the pollutant transformation behaviors in sewers.