Abstract
A novel flow-injection chemiluminescence method for the determination of melamine in urine and plasma was developed. It was found that melamine can remarkably enhance chemiluminescence emission from the luminol-K 3Fe(CN)6 system in an alkaline medium. Under the optimum conditions, chemiluminescence intensity had a good linear relationship with the concentration of melamine in the range 9.0 × 10-9-7.0 × 10-6 g/mL, with a correlation coefficient of 0.9992. The detection limit (3σ) was 3.5 ng/mL. The method has been applied to determine the concentration of melamine in samples using liquid-liquid extraction. Average recoveries of melamine were 102.6% in urine samples and 95.1% in plasma samples. The method provided a reproducible and stable approach for the sensitive detection of melamine in urine and plasma samples.
| Original language | English |
|---|---|
| Pages (from-to) | 229-233 |
| Number of pages | 5 |
| Journal | Luminescence |
| Volume | 27 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2012 |
| Externally published | Yes |
Keywords
- chemiluminescence
- flow-injection analysis
- melamine
- plasma
- urine