Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer

Min Lin; Yan Gao; Thomas J. Diefenbach; Jacson K. Shen; Francis J. Hornicek; Yong Il Park; Feng Xu; Tian Jian Lu; Mansoor Amiji; Zhenfeng Duan

doi:10.1021/acsami.6b15321

Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer

Min Lin
, Yan Gao
, Thomas J. Diefenbach
, Jacson K. Shen
, Francis J. Hornicek
, Yong Il Park
, Feng Xu
, Tian Jian Lu
, Mansoor Amiji
, Zhenfeng Duan

School of Life Science and Technology (SLST)

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Development of multidrug resistance (MDR) contributes to the majority of treatment failures in clinical chemotherapy. We report facial layer-by-layer engineered upconversion nanoparticles (UCNPs) for near-infrared (NIR)-initiated tracking and delivery of small interfering RNA (siRNA) to enhance chemotherapy efficacy by silencing the MDR1 gene and resensitizing resistant ovarian cancer cells to drug. Layer-by-layer engineered UCNPs were loaded with MDR1 gene-silencing siRNA (MDR1-siRNA) by electrostatic interaction. The delivery vehicle enhances MDR1-siRNA cellular uptake, protects MDR1-siRNA from nuclease degradation, and promotes endosomal escape for silencing the MDR gene. The intrinsic photon upconversion of UCNPs provides an unprecedented opportunity for monitoring intracellular attachment and release of MDR1-siRNA by NIR-initiated fluorescence resonance energy transfer occurs between donor UCNPs and acceptor fluorescence dye-labeled MDR1-siRNA. Enhanced chemotherapeutic efficacy in vitro was demonstrated by cell viability assay. The developed delivery vehicle holds great potential in delivery and imaging-guided tracking of therapeutic gene targets for effective treatment of drug-resistant cancers.

Original language	English
Pages (from-to)	7941-7949
Number of pages	9
Journal	ACS Applied Materials and Interfaces
Volume	9
Issue number	9
DOIs	https://doi.org/10.1021/acsami.6b15321
State	Published - 8 Mar 2017

Keywords

FRET
drug sensitivity
gene delivery
gene knockdown
upconversion nanoparticles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsami.6b15321

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Lin, M., Gao, Y., Diefenbach, T. J., Shen, J. K., Hornicek, F. J., Park, Y. I., Xu, F., Lu, T. J., Amiji, M., & Duan, Z. (2017). Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer. ACS Applied Materials and Interfaces, 9(9), 7941-7949. https://doi.org/10.1021/acsami.6b15321

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title = "Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer",

abstract = "Development of multidrug resistance (MDR) contributes to the majority of treatment failures in clinical chemotherapy. We report facial layer-by-layer engineered upconversion nanoparticles (UCNPs) for near-infrared (NIR)-initiated tracking and delivery of small interfering RNA (siRNA) to enhance chemotherapy efficacy by silencing the MDR1 gene and resensitizing resistant ovarian cancer cells to drug. Layer-by-layer engineered UCNPs were loaded with MDR1 gene-silencing siRNA (MDR1-siRNA) by electrostatic interaction. The delivery vehicle enhances MDR1-siRNA cellular uptake, protects MDR1-siRNA from nuclease degradation, and promotes endosomal escape for silencing the MDR gene. The intrinsic photon upconversion of UCNPs provides an unprecedented opportunity for monitoring intracellular attachment and release of MDR1-siRNA by NIR-initiated fluorescence resonance energy transfer occurs between donor UCNPs and acceptor fluorescence dye-labeled MDR1-siRNA. Enhanced chemotherapeutic efficacy in vitro was demonstrated by cell viability assay. The developed delivery vehicle holds great potential in delivery and imaging-guided tracking of therapeutic gene targets for effective treatment of drug-resistant cancers.",

keywords = "FRET, drug sensitivity, gene delivery, gene knockdown, upconversion nanoparticles",

author = "Min Lin and Yan Gao and Diefenbach, \{Thomas J.\} and Shen, \{Jacson K.\} and Hornicek, \{Francis J.\} and Park, \{Yong Il\} and Feng Xu and Lu, \{Tian Jian\} and Mansoor Amiji and Zhenfeng Duan",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = mar,

day = "8",

doi = "10.1021/acsami.6b15321",

language = "英语",

volume = "9",

pages = "7941--7949",

journal = "ACS Applied Materials and Interfaces",

issn = "1944-8244",

publisher = "American Chemical Society",

number = "9",

}

Lin, M, Gao, Y, Diefenbach, TJ, Shen, JK, Hornicek, FJ, Park, YI, Xu, F, Lu, TJ, Amiji, M & Duan, Z 2017, 'Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer', ACS Applied Materials and Interfaces, vol. 9, no. 9, pp. 7941-7949. https://doi.org/10.1021/acsami.6b15321

Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer. / Lin, Min; Gao, Yan; Diefenbach, Thomas J. et al.
In: ACS Applied Materials and Interfaces, Vol. 9, No. 9, 08.03.2017, p. 7941-7949.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery

T2 - Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer

AU - Lin, Min

AU - Gao, Yan

AU - Diefenbach, Thomas J.

AU - Shen, Jacson K.

AU - Hornicek, Francis J.

AU - Park, Yong Il

AU - Xu, Feng

AU - Lu, Tian Jian

AU - Amiji, Mansoor

AU - Duan, Zhenfeng

PY - 2017/3/8

Y1 - 2017/3/8

N2 - Development of multidrug resistance (MDR) contributes to the majority of treatment failures in clinical chemotherapy. We report facial layer-by-layer engineered upconversion nanoparticles (UCNPs) for near-infrared (NIR)-initiated tracking and delivery of small interfering RNA (siRNA) to enhance chemotherapy efficacy by silencing the MDR1 gene and resensitizing resistant ovarian cancer cells to drug. Layer-by-layer engineered UCNPs were loaded with MDR1 gene-silencing siRNA (MDR1-siRNA) by electrostatic interaction. The delivery vehicle enhances MDR1-siRNA cellular uptake, protects MDR1-siRNA from nuclease degradation, and promotes endosomal escape for silencing the MDR gene. The intrinsic photon upconversion of UCNPs provides an unprecedented opportunity for monitoring intracellular attachment and release of MDR1-siRNA by NIR-initiated fluorescence resonance energy transfer occurs between donor UCNPs and acceptor fluorescence dye-labeled MDR1-siRNA. Enhanced chemotherapeutic efficacy in vitro was demonstrated by cell viability assay. The developed delivery vehicle holds great potential in delivery and imaging-guided tracking of therapeutic gene targets for effective treatment of drug-resistant cancers.

AB - Development of multidrug resistance (MDR) contributes to the majority of treatment failures in clinical chemotherapy. We report facial layer-by-layer engineered upconversion nanoparticles (UCNPs) for near-infrared (NIR)-initiated tracking and delivery of small interfering RNA (siRNA) to enhance chemotherapy efficacy by silencing the MDR1 gene and resensitizing resistant ovarian cancer cells to drug. Layer-by-layer engineered UCNPs were loaded with MDR1 gene-silencing siRNA (MDR1-siRNA) by electrostatic interaction. The delivery vehicle enhances MDR1-siRNA cellular uptake, protects MDR1-siRNA from nuclease degradation, and promotes endosomal escape for silencing the MDR gene. The intrinsic photon upconversion of UCNPs provides an unprecedented opportunity for monitoring intracellular attachment and release of MDR1-siRNA by NIR-initiated fluorescence resonance energy transfer occurs between donor UCNPs and acceptor fluorescence dye-labeled MDR1-siRNA. Enhanced chemotherapeutic efficacy in vitro was demonstrated by cell viability assay. The developed delivery vehicle holds great potential in delivery and imaging-guided tracking of therapeutic gene targets for effective treatment of drug-resistant cancers.

KW - FRET

KW - drug sensitivity

KW - gene delivery

KW - gene knockdown

KW - upconversion nanoparticles

UR - https://www.scopus.com/pages/publications/85014971659

U2 - 10.1021/acsami.6b15321

DO - 10.1021/acsami.6b15321

M3 - 文章

C2 - 28177223

AN - SCOPUS:85014971659

SN - 1944-8244

VL - 9

SP - 7941

EP - 7949

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 9

ER -

Facial Layer-by-Layer Engineering of Upconversion Nanoparticles for Gene Delivery: Near-Infrared-Initiated Fluorescence Resonance Energy Transfer Tracking and Overcoming Drug Resistance in Ovarian Cancer

Abstract

Keywords

UN SDGs

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