Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite

Wei Wang; Hong Yi Li; De Wen Zhang; Jing Jiang; Yi Ran Cui; Shuang Qiu; Ying Lin Zhou; Xin Xiang Zhang

doi:10.1002/elan.201000235

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite

Wei Wang
, Hong Yi Li
, De Wen Zhang
, Jing Jiang
, Yi Ran Cui
, Shuang Qiu
, Ying Lin Zhou
, Xin Xiang Zhang

Peking University

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 μM with a linear range from 10 μM to 400 μM. The biosensor was successfully applied to the determination of glucose in human blood samples.

Original language	English
Pages (from-to)	2543-2550
Number of pages	8
Journal	Electroanalysis
Volume	22
Issue number	21
DOIs	https://doi.org/10.1002/elan.201000235
State	Published - Nov 2010
Externally published	Yes

Keywords

Au-BC nanocomposite
Biosensors
Glucose
Glucose oxidase
Horseradish peroxidase

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10.1002/elan.201000235

Cite this

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title = "Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite",

abstract = "An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 μM with a linear range from 10 μM to 400 μM. The biosensor was successfully applied to the determination of glucose in human blood samples.",

keywords = "Au-BC nanocomposite, Biosensors, Glucose, Glucose oxidase, Horseradish peroxidase",

author = "Wei Wang and Li, \{Hong Yi\} and Zhang, \{De Wen\} and Jing Jiang and Cui, \{Yi Ran\} and Shuang Qiu and Zhou, \{Ying Lin\} and Zhang, \{Xin Xiang\}",

year = "2010",

month = nov,

doi = "10.1002/elan.201000235",

language = "英语",

volume = "22",

pages = "2543--2550",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "21",

}

TY - JOUR

T1 - Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite

AU - Wang, Wei

AU - Li, Hong Yi

AU - Zhang, De Wen

AU - Jiang, Jing

AU - Cui, Yi Ran

AU - Qiu, Shuang

AU - Zhou, Ying Lin

AU - Zhang, Xin Xiang

PY - 2010/11

Y1 - 2010/11

N2 - An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 μM with a linear range from 10 μM to 400 μM. The biosensor was successfully applied to the determination of glucose in human blood samples.

AB - An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 μM with a linear range from 10 μM to 400 μM. The biosensor was successfully applied to the determination of glucose in human blood samples.

KW - Au-BC nanocomposite

KW - Biosensors

KW - Glucose

KW - Glucose oxidase

KW - Horseradish peroxidase

UR - https://www.scopus.com/pages/publications/78049247564

U2 - 10.1002/elan.201000235

DO - 10.1002/elan.201000235

M3 - 文章

AN - SCOPUS:78049247564

SN - 1040-0397

VL - 22

SP - 2543

EP - 2550

JO - Electroanalysis

JF - Electroanalysis

IS - 21

ER -

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles-Bacteria Cellulose Nanofibers Nanocomposite

Abstract

Keywords

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