Abstract
Objective: To detect the expression of NANOG in tumor tissue of human hepatocellular carcinoma and determine the effect of NANOG on cell invasion and migration in MHCC-97H cells by knockdown of NANOG. Methods: We collected 85 pairs of HCC and para-carcinoma tissues and detected the expression of NANOG by immunohistochemical staining. Clinicopathological and prognostic significance of NANOG was evaluated in these patients. The expression of NANOG was knocked down by siRNA in MHCC-97H cells. Transwell assays were performed to test HCC cell invasion and migration in vitro. Results: The expression of NANOG in HCC tissues was significantly higher than that in paired para-carcinoma tissues, and it was significantly associated with tumor size, TNM stage, portal vein infiltration and Edmondson degree. Patients in the higher NANOG group had a poorer 3-year survival than those in the lower group. When the expression of NANOG was downregulated by a specific siRNA, NANOG knockdown obviously inhibited cell invasion and migration in MHCC-97H cells. Conclusion: NANOG is overexpressed in HCC tissue and is correlated with the malignant clinicopathologic features. NANOG knockdown inhibits cell invasion and migration of HCC cells.
| Original language | English |
|---|---|
| Pages (from-to) | 684-688 |
| Number of pages | 5 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 37 |
| Issue number | 5 |
| DOIs | |
| State | Published - 5 Sep 2016 |
| Externally published | Yes |
Keywords
- Hepatocellular carcinoma
- Invasion
- Migration
- NANOG