TY - JOUR
T1 - Exploring exosome profiling via CytoFLEX Nano flow cytometer
T2 - Approaches and applications
AU - Cao, Li
AU - Wang, Hui
AU - Zhu, Jiaqi
AU - Li, Wen
AU - Han, Lin
AU - Wang, Zhuanzhuan
AU - Wang, Xiaofei
AU - Guo, Bo
AU - Ding, Jiangbo
AU - Tong, Dongdong
AU - Huang, Chen
N1 - Publisher Copyright:
© 2025 The Author(s). VIEW published by Shanghai Fuji Technology Consulting Co., Ltd, authorized by China Professional Community of Experimental Medicine, National Association Health Industry Enterprise Management (CPCEM) and John Wiley & Sons Australia, Ltd.
PY - 2025/12
Y1 - 2025/12
N2 - Exosomes, a subtype of extracellular vesicles, have garnered significant attention as biomarkers and therapeutic targets. Recent advances in flow cytometry have enhanced the characterization of individual particles and improved the analysis of extracellular vesicle populations. This study focused on the CytoFLEX Nano flow cytometer, designed for small particle detection. We assessed the particle size distribution and marker protein profiles of exosome samples, comparing those from different sources and isolated using various methods to examine the stability and heterogeneity of exosome markers. The results revealed that the CytoFLEX Nano effectively characterized exosome samples, differentiated those from various sources, and emphasized the considerable influence of different separation methods on exosome labeling and enrichment efficiency. Moreover, the CytoFLEX Nano exhibited higher sensitivity and better labeling efficiency than the CytoFLEX for detecting smaller particles. In conclusion, these findings help optimize instrument selection and experimental design, thereby supporting their broad application in fundamental research, disease monitoring, and clinical diagnostics.
AB - Exosomes, a subtype of extracellular vesicles, have garnered significant attention as biomarkers and therapeutic targets. Recent advances in flow cytometry have enhanced the characterization of individual particles and improved the analysis of extracellular vesicle populations. This study focused on the CytoFLEX Nano flow cytometer, designed for small particle detection. We assessed the particle size distribution and marker protein profiles of exosome samples, comparing those from different sources and isolated using various methods to examine the stability and heterogeneity of exosome markers. The results revealed that the CytoFLEX Nano effectively characterized exosome samples, differentiated those from various sources, and emphasized the considerable influence of different separation methods on exosome labeling and enrichment efficiency. Moreover, the CytoFLEX Nano exhibited higher sensitivity and better labeling efficiency than the CytoFLEX for detecting smaller particles. In conclusion, these findings help optimize instrument selection and experimental design, thereby supporting their broad application in fundamental research, disease monitoring, and clinical diagnostics.
KW - CytoFLEX Nano flow cytometry
KW - exosome
KW - exosome characterization
KW - extracellular vesicle flow cytometry
KW - surface biomarker analysis
UR - https://www.scopus.com/pages/publications/105017302353
U2 - 10.1002/VIW.20250068
DO - 10.1002/VIW.20250068
M3 - 文章
AN - SCOPUS:105017302353
SN - 2688-3988
VL - 6
JO - VIEW
JF - VIEW
IS - 6
M1 - 20250068
ER -