Experimental and numerical studies on liquid wicking into filter papers for paper-based diagnostics

Paper-based diagnostics have shown promising potential applications in human disease surveillance and food safety analysis at the point-of-care (POC). The liquid wicking behavior in diagnostic fibrous paper plays an important role in development of paper-based diagnostics. In the current study, we performed experimental and numerical research on the liquid wicking height and mass with three width strips into filter paper. The effective porosity could be conveniently measured in the light of the linear correlation between wicking height and mass by the experimental system. A modified model with considering evaporation effect was proposed to predict wicking height and mass. The predicted wicking height and mass using the evaporation model was much closer to the experimental data compared with the model without evaporation. The wicking speed initially decreased significantly and then maintained at a constant value at lower level. The evaporation effect tends to reduce the wicking flow speed. More wicking mass could be obtained at larger strip width but the corresponding reagent loss became significant. The proposed model with evaporation paved a way to understanding the fundamental of fluid flow in diagnostic paper and was essential to provide meaningful and useful reference for the research and development of paper-based diagnostics devices.