TY - JOUR
T1 - Exact analysis of ligand-induced dimerization of monomeric receptors
AU - Mack, Eric T.
AU - Perez-Castillejos, Raquel
AU - Suo, Zhigang
AU - Whitesides, George M.
PY - 2008/7/15
Y1 - 2008/7/15
N2 - This paper analyzes the equilibria involved in the dimerization of monomelic receptors with homo-bifunctional ligands. We provide analytical expressions that can be used to estimate the concentration of each species present in a mixture of homo-bifunctional ligand and monomelic proteins, given initial conditions defining the total concentration of bivalent ligand [L 2]0, the total concentration of protein [P]0, one dissociation constant Kd, and a parameter to account for cooperativity α. We demonstrate that the fraction of protein present in a complex of two proteins and one bivalent ligand (P·L2· P) is maximized at [L2]0 = Kd/2 + [P] 0/2.
AB - This paper analyzes the equilibria involved in the dimerization of monomelic receptors with homo-bifunctional ligands. We provide analytical expressions that can be used to estimate the concentration of each species present in a mixture of homo-bifunctional ligand and monomelic proteins, given initial conditions defining the total concentration of bivalent ligand [L 2]0, the total concentration of protein [P]0, one dissociation constant Kd, and a parameter to account for cooperativity α. We demonstrate that the fraction of protein present in a complex of two proteins and one bivalent ligand (P·L2· P) is maximized at [L2]0 = Kd/2 + [P] 0/2.
UR - https://www.scopus.com/pages/publications/49049108184
U2 - 10.1021/ac800578w
DO - 10.1021/ac800578w
M3 - 文章
C2 - 18543951
AN - SCOPUS:49049108184
SN - 0003-2700
VL - 80
SP - 5550
EP - 5555
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 14
ER -