TY - JOUR
T1 - Ethylene diamine tetraacetic acid-induced colonic crypt cell proliferation in rats
AU - Ma, Qing Yong
AU - Williamson, Kate E.
AU - Rowlands, Brian J.
PY - 2004/1/15
Y1 - 2004/1/15
N2 - Aim: To investigate the effect of ethylene diamine tetraacetic acid (EDTA) on proliferation of rat colonic cells. Methods: EDTA was administered into Wistar rats, carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in rats was studied with immunohistochemistry. Results: Marked regional differences in cell proliferation were found in all groups. In EDTA-treated animals, total labelling indexes in both proximal (10.00±0.44 vs 7.20±0.45) and distal (11.05±0.45 vs 8.65±0.34) colon and proliferate zone size (21.67±1.13 vs 16.75±1.45, 27.73±1.46 vs 21.74±1.07) were significantly higher than that in normal controls (P<0.05) and lower than that in DMH group (10.00±0.44 vs 11.54±0.45, 11.05±0.45 vs 13.13±0.46, 21.67±1.13 vs 35.52±1.58, 27.73±1.46 vs 39.61±1.32, P<0.05). Cumulative frequency distributions showed a shift of the EDTA distal curve to the right (P<0.05) while the EDTA proximal curve did not change compared to normal controls. Despite the changes of proliferative parameters, tumours did not develop in EDTA treated animals. Conclusion: Hyperproliferation appears to be more easily induced by EDTA in distal colon than in proximal colon. Hyperproliferation may need to exceed a threshold to develop colonic tumours. EDTA may work as a co-factor in colonic tumorigenesis.
AB - Aim: To investigate the effect of ethylene diamine tetraacetic acid (EDTA) on proliferation of rat colonic cells. Methods: EDTA was administered into Wistar rats, carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in rats was studied with immunohistochemistry. Results: Marked regional differences in cell proliferation were found in all groups. In EDTA-treated animals, total labelling indexes in both proximal (10.00±0.44 vs 7.20±0.45) and distal (11.05±0.45 vs 8.65±0.34) colon and proliferate zone size (21.67±1.13 vs 16.75±1.45, 27.73±1.46 vs 21.74±1.07) were significantly higher than that in normal controls (P<0.05) and lower than that in DMH group (10.00±0.44 vs 11.54±0.45, 11.05±0.45 vs 13.13±0.46, 21.67±1.13 vs 35.52±1.58, 27.73±1.46 vs 39.61±1.32, P<0.05). Cumulative frequency distributions showed a shift of the EDTA distal curve to the right (P<0.05) while the EDTA proximal curve did not change compared to normal controls. Despite the changes of proliferative parameters, tumours did not develop in EDTA treated animals. Conclusion: Hyperproliferation appears to be more easily induced by EDTA in distal colon than in proximal colon. Hyperproliferation may need to exceed a threshold to develop colonic tumours. EDTA may work as a co-factor in colonic tumorigenesis.
UR - https://www.scopus.com/pages/publications/1042292687
U2 - 10.3748/wjg.v10.i2.218
DO - 10.3748/wjg.v10.i2.218
M3 - 文章
C2 - 14716826
AN - SCOPUS:1042292687
SN - 1007-9327
VL - 10
SP - 218
EP - 222
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 2
ER -