Establishment of Metrigel-VEGF-SW982 complexes and application of the complexes in synovial sarcoma model in mice

Objective: To establish Metrigel-VEGF-SW982 complexes and use the complexes to produce animal models of synovial sarcoma so as to provide new ideas for establishing other models of soft tissue tumors. Methods: After the SW982 cells were cultured and collected, they were resuspended with Metrigel, and VEGF was added. The suspension was seeded into transwell to establish the scaffold complexes of Metrigel-VEGF-SW982. The complexes were cultured overnight. Cryosections were made and HE staining was carried out to observe the cell scaffold complexes. We randomly divided 10 female SCID mice (4 week old) into scaffold group and control group. The mice in the scaffold group were transplanted with cell-scaffold complexes, and the control group with cell suspension. After 8 weeks, the success rate of modeling was compared between two groups. The mice were sacrificed and the tumors were obtained. HE staining was carried out to observe the histopathological features of tumors in both scaffold group and control group. Results: The SW982 cells were cultured with Matrigel in a 3D way, which could simulate the growth condition of cells in vivo. Establishing synovial sarcoma animal model with cell scaffold complex could increase the success rate. The tumors in scaffold group had a larger volume, higher density of tumor cells and greater vascularization (P<0.05). Conclusion: Establishing synovial sarcoma animal model with Metrigel-VEGF-SW982 complex can greatly improve the success rate of modeling, which can provide basis for the study of synovial sarcoma.