TY - JOUR
T1 - Enhancement on hydrogen production performance of Rhodobacter sphaeroides HY01 by overexpressing fdxN
AU - Ma, Hongyu
AU - Zheng, Xiaojing
AU - Yang, Honghui
N1 - Publisher Copyright:
© 2018 Hydrogen Energy Publications LLC
PY - 2018/9/6
Y1 - 2018/9/6
N2 - Ferredoxin I (FdI), encoded by fdxN gene, is proved to be the main electron donor of nitrogenase for hydrogen production. In this work, fdxN gene overexpression was implemented in a mutant MHY01, which was constructed by inserting fdxN gene into the hupSL region in Rhodobacter sphaeroides HY01 genome. Its photo-fermentative H2 production performance was studied. The results showed that the expression level of fdxN and nitrogenase activity in MHY01 (hupSL::fdxN) were enhanced by 177% and 61.7% respectively compared with that of wild type HY01. Using 25 mM acetate and 34 mM butyrate as carbon source and 6 mM L-glutamate as nitrogen source, the maximum H2 production rate was 156.1 mL/(L·h), which was increased by 50.7% compared with that of HY01. The maximum H2 production rates of MHY01 were enhanced by 30.0%, 52.5% and 50.7% compared with those obtained from HY01 at the inoculation size of 5%, 10% and 15% respectively. The results suggested that overexpressing fdxN could enhance the nitrogenase activity and H2 production performance of purple non-sulfur bacteria. The abundancy of ferredoxin I might limit the efficiency of electron transfer flux associated with the biohydrogen production process.
AB - Ferredoxin I (FdI), encoded by fdxN gene, is proved to be the main electron donor of nitrogenase for hydrogen production. In this work, fdxN gene overexpression was implemented in a mutant MHY01, which was constructed by inserting fdxN gene into the hupSL region in Rhodobacter sphaeroides HY01 genome. Its photo-fermentative H2 production performance was studied. The results showed that the expression level of fdxN and nitrogenase activity in MHY01 (hupSL::fdxN) were enhanced by 177% and 61.7% respectively compared with that of wild type HY01. Using 25 mM acetate and 34 mM butyrate as carbon source and 6 mM L-glutamate as nitrogen source, the maximum H2 production rate was 156.1 mL/(L·h), which was increased by 50.7% compared with that of HY01. The maximum H2 production rates of MHY01 were enhanced by 30.0%, 52.5% and 50.7% compared with those obtained from HY01 at the inoculation size of 5%, 10% and 15% respectively. The results suggested that overexpressing fdxN could enhance the nitrogenase activity and H2 production performance of purple non-sulfur bacteria. The abundancy of ferredoxin I might limit the efficiency of electron transfer flux associated with the biohydrogen production process.
KW - Ferredoxin I
KW - Hydrogen production
KW - Nitrogenase activity
KW - fdxN overexpression
UR - https://www.scopus.com/pages/publications/85050937115
U2 - 10.1016/j.ijhydene.2018.07.101
DO - 10.1016/j.ijhydene.2018.07.101
M3 - 文章
AN - SCOPUS:85050937115
SN - 0360-3199
VL - 43
SP - 17082
EP - 17090
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 36
ER -
