Electrostatic detection of unlabelled single- and double-stranded DNA using capacitive field-effect devices functionalized with a positively charged polyelectrolyte layer

T. S. Bronder; A. Poghossian; S. Scheja; C. S. Wu; M. Keusgen; M. J. Schöning

doi:10.1016/j.proeng.2015.08.710

Electrostatic detection of unlabelled single- and double-stranded DNA using capacitive field-effect devices functionalized with a positively charged polyelectrolyte layer

T. S. Bronder
, A. Poghossian
, S. Scheja
, C. S. Wu
, M. Keusgen
, M. J. Schöning

Aachen University of Applied Sciences
Jülich Research Centre
Key Lab of the Ministry of Education for Process Control and Efficiency Egineering
University of Marburg

Research output: Contribution to journal › Conference article › peer-review

4 Scopus citations

Abstract

Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO₂ structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.

Original language	English
Pages (from-to)	544-547
Number of pages	4
Journal	Procedia Engineering
Volume	120
DOIs	https://doi.org/10.1016/j.proeng.2015.08.710
State	Published - 2015
Externally published	Yes
Event	29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015. - Freiburg, Germany Duration: 6 Sep 2015 → 9 Sep 2015

Keywords

DNA biosensor
Field-effect sensor
Label-free detection
Polyelectrolyte

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10.1016/j.proeng.2015.08.710

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title = "Electrostatic detection of unlabelled single- and double-stranded DNA using capacitive field-effect devices functionalized with a positively charged polyelectrolyte layer",

abstract = "Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.",

keywords = "DNA biosensor, Field-effect sensor, Label-free detection, Polyelectrolyte",

author = "Bronder, \{T. S.\} and A. Poghossian and S. Scheja and Wu, \{C. S.\} and M. Keusgen and Sch{\"o}ning, \{M. J.\}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors. Published by Elsevier Ltd.; 29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015. ; Conference date: 06-09-2015 Through 09-09-2015",

year = "2015",

doi = "10.1016/j.proeng.2015.08.710",

language = "英语",

volume = "120",

pages = "544--547",

journal = "Procedia Engineering",

issn = "1877-7058",

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T1 - Electrostatic detection of unlabelled single- and double-stranded DNA using capacitive field-effect devices functionalized with a positively charged polyelectrolyte layer

AU - Bronder, T. S.

AU - Poghossian, A.

AU - Scheja, S.

AU - Wu, C. S.

AU - Keusgen, M.

AU - Schöning, M. J.

PY - 2015

Y1 - 2015

N2 - Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.

AB - Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.

KW - DNA biosensor

KW - Field-effect sensor

KW - Label-free detection

KW - Polyelectrolyte

UR - https://www.scopus.com/pages/publications/84984985762

U2 - 10.1016/j.proeng.2015.08.710

DO - 10.1016/j.proeng.2015.08.710

M3 - 会议文章

AN - SCOPUS:84984985762

SN - 1877-7058

VL - 120

SP - 544

EP - 547

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 29th European Conference on Solid-State Transducers, EUROSENSORS 2015; Freiburg; Germany; 6 September 2015 through 9 September 2015.

Y2 - 6 September 2015 through 9 September 2015

ER -

Electrostatic detection of unlabelled single- and double-stranded DNA using capacitive field-effect devices functionalized with a positively charged polyelectrolyte layer

Abstract

Keywords

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