Effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro

Objective: To investigate the effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro. Methods: The routinely cultured human pancreatic cancer Miapaca-2 cells in vitro were treated with resveratrol of three different concentrations (200, 100 and 50 μmol/L). Then the effects of resveratrol on invasion and metastasis of pancreatic cancer Miapaca-2 cells in vitro were detected by adhesion, invasion and migration assays. The expressions of MMP-7 and MMP-9 at the protein and mRNA levels in the cells were measured by Western blot and RT-PCR. Results: The effects of resveratrol on adhesion, invasion and migration of pancreatic cancer Miapaca-2 cells were decreased in a concentration-dependent manner compared with those in the control group, and the difference was significant (P<0.01). Compared with those in negative control group, the expressions of MMP-7 and MMP-9 at the protein and mRNA levels in pancreatic cancer Miapaca-2 cells treated with resveratrol of three different concentrations were decreased in a concentration-dependent manner, and the difference was significant (P<0.01). Conclusion: Resveratrol in vitro can remarkably restrain the invasion and migration of pancreatic cancer Miapaca-2 cells in a concentration-dependent manner. The mechanism may be related to down-regulating the expressions of MMP-7 and MMP-9.