Abstract
Objective: To investigate the effects of resvertrol on apoptosis and the expression of apoptosis-regulated genes Bax in rats with experimental severe acute pancreatisis. Methods: Totally 54 SD rats were randomly divided into 3 groups: sham-operation group (SO) , severe acute pancreatitis group (SAP), and resveratrol-treated group (RES). In SO group the pancreases were slightly flipped only. The SAP model was set up by giving 40 g/L sodium chrolate (1 mL/kg) through pancreatic duct, and resveratrol (5 mg/mL, 10 mg/kg) was given intravenously. Apoptosis of mucosal cells was detected by TUNEL methods, and the expression of Bax protein was determined by SP immunohistochemical technique. Results: The apoptotic index of mucosal cells in SAP group at 3, 6 and 12 hours after induction of severe acute pancreatitis was significantly higher than that in resveratrol-treated group (P<0.01). The positive rate in resveratrol-treated group at 3, 6 and 12 hours was significantly lower than that in SAP group (P<0.01). Conclusion: Restrainting apoptotic intestinal mucosa cells and defending the intestinal barrier function from injury might be one of the mechanisms of resvertrol in treating severe acute pancreatitis.
| Original language | English |
|---|---|
| Pages (from-to) | 593-595 |
| Number of pages | 3 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 27 |
| Issue number | 6 |
| State | Published - Dec 2006 |
Keywords
- Apoptosis
- Bax protein
- Resveratrol
- Severe acute pancreatitis