Effect of taspine on wound healing and fibroblast proliferation

Objective: To study the effect and mechanism of taspine on wound healing and fibroblast proliferation. Methods: The effect of taspine on skin wound was observed in vivo. The different concentration of taspine hydrochloride was added to L₉₂₉ fibroblast cultivated in vitro, and lactate dehydrogenase was detected and MTT method was applied to observe effect of taspine on fibroblast proliferation. Results: The local application of taspine 3 mg/mL and 1.5 mg/mL accelerated the healing of skin wounded. In vitro, 0.01-0.5 μg/mL of taspine hydrochloride showed no effect on the change of lactate dehydrogenase activity and fibroblast proliferation. Conclusion: Taspine is a kind of active alkaloid from leontice robustum which can enhance wound healing, its mechanism on wound healing is not by means of accelerating the proliferation of fibroblast, other mechanisms are necessary for being further studied.