Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro

Ya Lin Dong; Lang Chong He; Fang Chen

Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro

Ya Lin Dong
, Lang Chong He
, Fang Chen

Health Science Center

Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

OBJECTIVE: To investigate the mechanism of enhancing wound healing by taspine hydrochloride (TA). METHODS: Fibroblasts were cultured in vitro, and TA was applied with different concentrations (0.05,0.1,0.2,0.4 and 0.5 μg·mL^-1) and time (24,48 and 72 h) to influence their autocrine. The levels of transforming growth factor-β₁ (TGF-β₁) and epidermal growth factor (EGF) were determined by enzyme-linked immunoadsorbent assay (ELIAS). RESULTS: TA increased autocrine of TGF-β₁ and EGF by fibroblasts, the action was reinforced with the increasing TA concentration and the extending of time. CONCLUSION: The above results indicated that TA increased autocrining of TGF-β₁ and EGF, and accelerated the chemotaxis of fibroblast and inflammation cell, the deposition of extracellular matrix and wound healing.

Original language	English
Pages (from-to)	826-828
Number of pages	3
Journal	Chinese Pharmaceutical Journal
Volume	40
Issue number	11
State	Published - Jun 2005

Keywords

Epidermal growth factor
Taspine hydrochloride
Transforming growth factor-β
Wound healing

Cite this

@article{d0b0266630014bd6be563db72230a700,

title = "Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro",

abstract = "OBJECTIVE: To investigate the mechanism of enhancing wound healing by taspine hydrochloride (TA). METHODS: Fibroblasts were cultured in vitro, and TA was applied with different concentrations (0.05,0.1,0.2,0.4 and 0.5 μg·mL-1) and time (24,48 and 72 h) to influence their autocrine. The levels of transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) were determined by enzyme-linked immunoadsorbent assay (ELIAS). RESULTS: TA increased autocrine of TGF-β1 and EGF by fibroblasts, the action was reinforced with the increasing TA concentration and the extending of time. CONCLUSION: The above results indicated that TA increased autocrining of TGF-β1 and EGF, and accelerated the chemotaxis of fibroblast and inflammation cell, the deposition of extracellular matrix and wound healing.",

keywords = "Epidermal growth factor, Taspine hydrochloride, Transforming growth factor-β, Wound healing",

author = "Dong, \{Ya Lin\} and He, \{Lang Chong\} and Fang Chen",

year = "2005",

month = jun,

language = "英语",

volume = "40",

pages = "826--828",

journal = "Chinese Pharmaceutical Journal",

issn = "1001-2494",

publisher = "Chinese Pharmaceutical Association",

number = "11",

}

TY - JOUR

T1 - Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro

AU - Dong, Ya Lin

AU - He, Lang Chong

AU - Chen, Fang

PY - 2005/6

Y1 - 2005/6

N2 - OBJECTIVE: To investigate the mechanism of enhancing wound healing by taspine hydrochloride (TA). METHODS: Fibroblasts were cultured in vitro, and TA was applied with different concentrations (0.05,0.1,0.2,0.4 and 0.5 μg·mL-1) and time (24,48 and 72 h) to influence their autocrine. The levels of transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) were determined by enzyme-linked immunoadsorbent assay (ELIAS). RESULTS: TA increased autocrine of TGF-β1 and EGF by fibroblasts, the action was reinforced with the increasing TA concentration and the extending of time. CONCLUSION: The above results indicated that TA increased autocrining of TGF-β1 and EGF, and accelerated the chemotaxis of fibroblast and inflammation cell, the deposition of extracellular matrix and wound healing.

AB - OBJECTIVE: To investigate the mechanism of enhancing wound healing by taspine hydrochloride (TA). METHODS: Fibroblasts were cultured in vitro, and TA was applied with different concentrations (0.05,0.1,0.2,0.4 and 0.5 μg·mL-1) and time (24,48 and 72 h) to influence their autocrine. The levels of transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF) were determined by enzyme-linked immunoadsorbent assay (ELIAS). RESULTS: TA increased autocrine of TGF-β1 and EGF by fibroblasts, the action was reinforced with the increasing TA concentration and the extending of time. CONCLUSION: The above results indicated that TA increased autocrining of TGF-β1 and EGF, and accelerated the chemotaxis of fibroblast and inflammation cell, the deposition of extracellular matrix and wound healing.

KW - Epidermal growth factor

KW - Taspine hydrochloride

KW - Transforming growth factor-β

KW - Wound healing

UR - https://www.scopus.com/pages/publications/23644445815

M3 - 文章

AN - SCOPUS:23644445815

SN - 1001-2494

VL - 40

SP - 826

EP - 828

JO - Chinese Pharmaceutical Journal

JF - Chinese Pharmaceutical Journal

IS - 11

ER -

Effect of taspine hydrochloride on autocrining growth factor of fibroblast in vitro

Abstract

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