Effect of SLC modification on the immunogenicity of tumor cell vaccine

OBJECTIVE: To construct SLC-modified chemo-tactic melanoma vaccine and investigate its function of immunogenicity. METHODS: Murine SLC gene was transfected into melanoma cells B16. Chemotactic melanoma vaccine was constructed from transfected cells treated with mytomycin C. In vitro, culture supernatant of chemotactic vaccine was collected and its chemotactic function to lymphocytes was detected by a chemotax-is chamber. In vivo, CTL activity of spleen cells from mice immunized with chemotactic vaccine was examined, and the effect on growth of melanoma was also detected. RESULTS: B16 melanoma cells transfected with SLC gene were picked out, and then were treated with 100 μg/mL mytomycin C for 1.5 hours. Such chemotactic vaccine were cultured for 48 hours, and chemotactic function to lymphocytes was detected from the culture supernatant. CTL activity of spleen cells from mice immunized with chemotactic vaccine was more effective than that from mice immunize with wild vaccine. The suppression on growth of melanoma was also more remarkable in the chemotactic vaccine group than that in the wild vaccine group. CONCLUSION: Chemotactic melanoma vaccine has been constructed and the effect of such vaccine on anti-tumor immunity and growth of melanoma is remarkable. Such vaccine could be used in immunotherepy on malignant melanoma.