Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons

L. N. Liu; F. P. Zhu; M. Y. Song; X. J. Kang; S. J. Shang; X. Y. Zhang; H. D. Xu; S. S. Teng; B. Liu; S. T. Kuo; W. Liu; M. L. Li; L. Zhou; R. Y. Jiao; C. H. Wang; S. R. Wang; H. Yang; B. Zhang; Z. Zhou; Z. Q.D. Xu

doi:10.1007/s12031-013-0159-5

Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons

L. N. Liu
, F. P. Zhu
, M. Y. Song
, X. J. Kang
, S. J. Shang
, X. Y. Zhang
, H. D. Xu
, S. S. Teng
, B. Liu
, S. T. Kuo
, W. Liu
, M. L. Li
, L. Zhou
, R. Y. Jiao
, C. H. Wang
, S. R. Wang
, H. Yang
, B. Zhang
, Z. Zhou
, Z. Q.D. Xu

Capital Medical University
State Key Lab. of Biomembr. and Membr. Biotechnol. and Beijing Key Lab. of Cardiometabolic Mol. Med., Institute of Molecular Medicine and Peking-Tsinghua Center for Life Sciences, PKU-IDG/McGovern Institute for Brain Research, Peking University
Jilin University
Peking University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The locus coeruleus (LC) is an important brainstem area involved in cocaine addiction. However, evidence to elucidate how cocaine modulates the activity of LC neurons remains incomplete. Here, we performed whole recordings in brain slices to evaluate the effects of cocaine on the sodium (Na⁺), potassium (K⁺), calcium (Ca²⁺) channels, and glutamatergic synaptic transmission in the locus coeruleus neurons. Local application of cocaine significantly and reversibly reduced the spontaneous firing rate but did not affect action potential amplitude, rising time, decay time, or half width of noradrenergic locus coeruleus neurons. Moreover, cocaine attenuated the sodium current but did not affect potassium and calcium currents. The N-methyl-d-aspartate receptor mediated excitatory postsynaptic currents were reduced by neuropeptide galanin but not cocaine. All those data demonstrate that cocaine has inhibitory effect on the spontaneous activities and sodium current in locus coeruleus neurons. Therefore, neuromodulation of sodium channel in locus coeruleus neurons may play an important role in drug addiction.

Original language	English
Pages (from-to)	345-351
Number of pages	7
Journal	Journal of Molecular Neuroscience
Volume	53
Issue number	3
DOIs	https://doi.org/10.1007/s12031-013-0159-5
State	Published - Jun 2014
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cocaine
EPSC
Galanin
Locus coeruleus brain slice
Voltage gated ion channels

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10.1007/s12031-013-0159-5

Cite this

Liu, L. N., Zhu, F. P., Song, M. Y., Kang, X. J., Shang, S. J., Zhang, X. Y., Xu, H. D., Teng, S. S., Liu, B., Kuo, S. T., Liu, W., Li, M. L., Zhou, L., Jiao, R. Y., Wang, C. H., Wang, S. R., Yang, H., Zhang, B., Zhou, Z., & Xu, Z. Q. D. (2014). Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons. Journal of Molecular Neuroscience, 53(3), 345-351. https://doi.org/10.1007/s12031-013-0159-5

@article{7964cea311c94784990127363c00d3f2,

title = "Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons",

abstract = "The locus coeruleus (LC) is an important brainstem area involved in cocaine addiction. However, evidence to elucidate how cocaine modulates the activity of LC neurons remains incomplete. Here, we performed whole recordings in brain slices to evaluate the effects of cocaine on the sodium (Na+), potassium (K+), calcium (Ca2+) channels, and glutamatergic synaptic transmission in the locus coeruleus neurons. Local application of cocaine significantly and reversibly reduced the spontaneous firing rate but did not affect action potential amplitude, rising time, decay time, or half width of noradrenergic locus coeruleus neurons. Moreover, cocaine attenuated the sodium current but did not affect potassium and calcium currents. The N-methyl-d-aspartate receptor mediated excitatory postsynaptic currents were reduced by neuropeptide galanin but not cocaine. All those data demonstrate that cocaine has inhibitory effect on the spontaneous activities and sodium current in locus coeruleus neurons. Therefore, neuromodulation of sodium channel in locus coeruleus neurons may play an important role in drug addiction.",

keywords = "Cocaine, EPSC, Galanin, Locus coeruleus brain slice, Voltage gated ion channels",

author = "Liu, \{L. N.\} and Zhu, \{F. P.\} and Song, \{M. Y.\} and Kang, \{X. J.\} and Shang, \{S. J.\} and Zhang, \{X. Y.\} and Xu, \{H. D.\} and Teng, \{S. S.\} and B. Liu and Kuo, \{S. T.\} and W. Liu and Li, \{M. L.\} and L. Zhou and Jiao, \{R. Y.\} and Wang, \{C. H.\} and Wang, \{S. R.\} and H. Yang and B. Zhang and Z. Zhou and Xu, \{Z. Q.D.\}",

year = "2014",

month = jun,

doi = "10.1007/s12031-013-0159-5",

language = "英语",

volume = "53",

pages = "345--351",

journal = "Journal of Molecular Neuroscience",

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Liu, LN, Zhu, FP, Song, MY, Kang, XJ, Shang, SJ, Zhang, XY, Xu, HD, Teng, SS, Liu, B, Kuo, ST, Liu, W, Li, ML, Zhou, L, Jiao, RY, Wang, CH, Wang, SR, Yang, H, Zhang, B, Zhou, Z & Xu, ZQD 2014, 'Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons', Journal of Molecular Neuroscience, vol. 53, no. 3, pp. 345-351. https://doi.org/10.1007/s12031-013-0159-5

TY - JOUR

T1 - Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons

AU - Liu, L. N.

AU - Zhu, F. P.

AU - Song, M. Y.

AU - Kang, X. J.

AU - Shang, S. J.

AU - Zhang, X. Y.

AU - Xu, H. D.

AU - Teng, S. S.

AU - Liu, B.

AU - Kuo, S. T.

AU - Liu, W.

AU - Li, M. L.

AU - Zhou, L.

AU - Jiao, R. Y.

AU - Wang, C. H.

AU - Wang, S. R.

AU - Yang, H.

AU - Zhang, B.

AU - Zhou, Z.

AU - Xu, Z. Q.D.

PY - 2014/6

Y1 - 2014/6

N2 - The locus coeruleus (LC) is an important brainstem area involved in cocaine addiction. However, evidence to elucidate how cocaine modulates the activity of LC neurons remains incomplete. Here, we performed whole recordings in brain slices to evaluate the effects of cocaine on the sodium (Na+), potassium (K+), calcium (Ca2+) channels, and glutamatergic synaptic transmission in the locus coeruleus neurons. Local application of cocaine significantly and reversibly reduced the spontaneous firing rate but did not affect action potential amplitude, rising time, decay time, or half width of noradrenergic locus coeruleus neurons. Moreover, cocaine attenuated the sodium current but did not affect potassium and calcium currents. The N-methyl-d-aspartate receptor mediated excitatory postsynaptic currents were reduced by neuropeptide galanin but not cocaine. All those data demonstrate that cocaine has inhibitory effect on the spontaneous activities and sodium current in locus coeruleus neurons. Therefore, neuromodulation of sodium channel in locus coeruleus neurons may play an important role in drug addiction.

AB - The locus coeruleus (LC) is an important brainstem area involved in cocaine addiction. However, evidence to elucidate how cocaine modulates the activity of LC neurons remains incomplete. Here, we performed whole recordings in brain slices to evaluate the effects of cocaine on the sodium (Na+), potassium (K+), calcium (Ca2+) channels, and glutamatergic synaptic transmission in the locus coeruleus neurons. Local application of cocaine significantly and reversibly reduced the spontaneous firing rate but did not affect action potential amplitude, rising time, decay time, or half width of noradrenergic locus coeruleus neurons. Moreover, cocaine attenuated the sodium current but did not affect potassium and calcium currents. The N-methyl-d-aspartate receptor mediated excitatory postsynaptic currents were reduced by neuropeptide galanin but not cocaine. All those data demonstrate that cocaine has inhibitory effect on the spontaneous activities and sodium current in locus coeruleus neurons. Therefore, neuromodulation of sodium channel in locus coeruleus neurons may play an important role in drug addiction.

KW - Cocaine

KW - EPSC

KW - Galanin

KW - Locus coeruleus brain slice

KW - Voltage gated ion channels

UR - https://www.scopus.com/pages/publications/84905006913

U2 - 10.1007/s12031-013-0159-5

DO - 10.1007/s12031-013-0159-5

M3 - 文章

C2 - 24214104

AN - SCOPUS:84905006913

SN - 0895-8696

VL - 53

SP - 345

EP - 351

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

IS - 3

ER -

Effect of cocaine on ion channels and glutamatergic EPSCs in noradrenergic locus coeruleus neurons

Abstract

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Keywords

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