Development of a microfluidic system for measuring HIV-1 viral load

Shuqi Wang; Alexander Ip; Feng Xu; Francoise F. Giguel; Sangjun Moon; Altug Akay; Daniel R. Kuritzkes; Utkan Demirci

doi:10.1117/12.853132

Development of a microfluidic system for measuring HIV-1 viral load

Shuqi Wang
, Alexander Ip
, Feng Xu
, Francoise F. Giguel
, Sangjun Moon
, Altug Akay
, Daniel R. Kuritzkes
, Utkan Demirci

Brigham and Women’s Hospital
Massachusetts General Hospital
Harvard-Massachusetts Institutes of Technology Health Sciences and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

12 Scopus citations

Abstract

The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.

Original language	English
Title of host publication	Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX
DOIs	https://doi.org/10.1117/12.853132
State	Published - 2010
Externally published	Yes
Event	Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX - Orlando, FL, United States Duration: 5 Apr 2010 → 9 Apr 2010

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	7666
ISSN (Print)	0277-786X

Conference

Conference	Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX
Country/Territory	United States
City	Orlando, FL
Period	5/04/10 → 9/04/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Human immunodeficiency virus type 1 (HIV-1)
microchip
point-of-care (POC)
viral load

Access to Document

10.1117/12.853132

Cite this

Wang, S., Ip, A., Xu, F., Giguel, F. F., Moon, S., Akay, A., Kuritzkes, D. R., & Demirci, U. (2010). Development of a microfluidic system for measuring HIV-1 viral load. In Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX Article 76661H (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7666). https://doi.org/10.1117/12.853132

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title = "Development of a microfluidic system for measuring HIV-1 viral load",

abstract = "The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.",

keywords = "Human immunodeficiency virus type 1 (HIV-1), microchip, point-of-care (POC), viral load",

author = "Shuqi Wang and Alexander Ip and Feng Xu and Giguel, \{Francoise F.\} and Sangjun Moon and Altug Akay and Kuritzkes, \{Daniel R.\} and Utkan Demirci",

year = "2010",

doi = "10.1117/12.853132",

language = "英语",

isbn = "9780819481306",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX",

note = "Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX ; Conference date: 05-04-2010 Through 09-04-2010",

}

Wang, S, Ip, A, Xu, F, Giguel, FF, Moon, S, Akay, A, Kuritzkes, DR & Demirci, U 2010, Development of a microfluidic system for measuring HIV-1 viral load. in Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX., 76661H, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX, Orlando, FL, United States, 5/04/10. https://doi.org/10.1117/12.853132

Development of a microfluidic system for measuring HIV-1 viral load. / Wang, Shuqi; Ip, Alexander; Xu, Feng et al.
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX. 2010. 76661H (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7666).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Development of a microfluidic system for measuring HIV-1 viral load

AU - Wang, Shuqi

AU - Ip, Alexander

AU - Xu, Feng

AU - Giguel, Francoise F.

AU - Moon, Sangjun

AU - Akay, Altug

AU - Kuritzkes, Daniel R.

AU - Demirci, Utkan

PY - 2010

Y1 - 2010

N2 - The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.

AB - The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.

KW - Human immunodeficiency virus type 1 (HIV-1)

KW - microchip

KW - point-of-care (POC)

KW - viral load

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DO - 10.1117/12.853132

M3 - 会议稿件

AN - SCOPUS:79957991929

SN - 9780819481306

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX

T2 - Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX

Y2 - 5 April 2010 through 9 April 2010

ER -

Development of a microfluidic system for measuring HIV-1 viral load

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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