Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue: Application to study its pharmacokinetics

Binyu Wen; Sicen Wang; Langchong He

doi:10.1080/00032711003763681

Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue: Application to study its pharmacokinetics

Binyu Wen
, Sicen Wang
, Langchong He

Health Science Center

Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A rapid and sensitive liquid chromatography-mass spectrometric (LC-MS) method was developed for investigating the pharmacokinetics of caulophine in rats. Biological samples were isolated by solid phase extraction (SPE) and analyzed by the LC-MS. A Shimadzu C18 VP-ODS column (150mm × 2.0mm ID, 5 μm) was used as the analytical column, with methanol-water-formic acid (33:67:0.1) as the mobile phase and a flow rate of 0.2mL/min. Within-day and between-day precision was 1.40-3.49% and 1.29-6.91%. Average recoveries for all matrices were greater than 85.0%. The main pharmacokinetic parameters were T_max=(0.84±0.31) h, C_max=(0.22±0.08) μg/mL, AUC=(0.87±0.16) h·μg/mL.

Original language	English
Pages (from-to)	2872-2882
Number of pages	11
Journal	Analytical Letters
Volume	43
Issue number	18
DOIs	https://doi.org/10.1080/00032711003763681
State	Published - Dec 2010

Keywords

Caulophine
LC-MS
Pharmacokinetics
Protein binding ratio
Tissue distribution

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10.1080/00032711003763681

Cite this

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title = "Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue: Application to study its pharmacokinetics",

abstract = "A rapid and sensitive liquid chromatography-mass spectrometric (LC-MS) method was developed for investigating the pharmacokinetics of caulophine in rats. Biological samples were isolated by solid phase extraction (SPE) and analyzed by the LC-MS. A Shimadzu C18 VP-ODS column (150mm × 2.0mm ID, 5 μm) was used as the analytical column, with methanol-water-formic acid (33:67:0.1) as the mobile phase and a flow rate of 0.2mL/min. Within-day and between-day precision was 1.40-3.49\% and 1.29-6.91\%. Average recoveries for all matrices were greater than 85.0\%. The main pharmacokinetic parameters were Tmax=(0.84±0.31) h, Cmax=(0.22±0.08) μg/mL, AUC=(0.87±0.16) h·μg/mL.",

keywords = "Caulophine, LC-MS, Pharmacokinetics, Protein binding ratio, Tissue distribution",

author = "Binyu Wen and Sicen Wang and Langchong He",

year = "2010",

month = dec,

doi = "10.1080/00032711003763681",

language = "英语",

volume = "43",

pages = "2872--2882",

journal = "Analytical Letters",

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TY - JOUR

T1 - Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue

T2 - Application to study its pharmacokinetics

AU - Wen, Binyu

AU - Wang, Sicen

AU - He, Langchong

PY - 2010/12

Y1 - 2010/12

N2 - A rapid and sensitive liquid chromatography-mass spectrometric (LC-MS) method was developed for investigating the pharmacokinetics of caulophine in rats. Biological samples were isolated by solid phase extraction (SPE) and analyzed by the LC-MS. A Shimadzu C18 VP-ODS column (150mm × 2.0mm ID, 5 μm) was used as the analytical column, with methanol-water-formic acid (33:67:0.1) as the mobile phase and a flow rate of 0.2mL/min. Within-day and between-day precision was 1.40-3.49% and 1.29-6.91%. Average recoveries for all matrices were greater than 85.0%. The main pharmacokinetic parameters were Tmax=(0.84±0.31) h, Cmax=(0.22±0.08) μg/mL, AUC=(0.87±0.16) h·μg/mL.

AB - A rapid and sensitive liquid chromatography-mass spectrometric (LC-MS) method was developed for investigating the pharmacokinetics of caulophine in rats. Biological samples were isolated by solid phase extraction (SPE) and analyzed by the LC-MS. A Shimadzu C18 VP-ODS column (150mm × 2.0mm ID, 5 μm) was used as the analytical column, with methanol-water-formic acid (33:67:0.1) as the mobile phase and a flow rate of 0.2mL/min. Within-day and between-day precision was 1.40-3.49% and 1.29-6.91%. Average recoveries for all matrices were greater than 85.0%. The main pharmacokinetic parameters were Tmax=(0.84±0.31) h, Cmax=(0.22±0.08) μg/mL, AUC=(0.87±0.16) h·μg/mL.

KW - Caulophine

KW - LC-MS

KW - Pharmacokinetics

KW - Protein binding ratio

KW - Tissue distribution

UR - https://www.scopus.com/pages/publications/78650226127

U2 - 10.1080/00032711003763681

DO - 10.1080/00032711003763681

M3 - 文章

AN - SCOPUS:78650226127

SN - 0003-2719

VL - 43

SP - 2872

EP - 2882

JO - Analytical Letters

JF - Analytical Letters

IS - 18

ER -

Development and validation of a solid phase extraction and LC-MS method for the determination of caulophine in rat plasma and tissue: Application to study its pharmacokinetics

Abstract

Keywords

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