TY - JOUR
T1 - Development and validation of a gas chromatography-mass spectrometry method for the determination of imperatorin in rat plasma and tissue
T2 - Application to study its pharmacokinetics
AU - Wang, Sicen
AU - Chen, Qinhua
AU - Wang, Lan
AU - He, Langchong
PY - 2009/7
Y1 - 2009/7
N2 - A rapid and sensitive assay for the quantification of imperatorin in plasma and tissues has been developed. An analysis was performed by gas chromatography/mass spectrometry in the selected ion-monitoring mode. The main pharmacokinetic parameters obtained were T max = 1.23 ± 0.26 h, C max = 0.95 ± 0.38 μg/mL, AUC = 3.42 ± 0.52 h μg/mL and K a = 1.34 ± 0.18 h. The experimental results showed that imperatorin was easily absorbed, but its elimination was slow, from 3 to 12 h after oral administration. The concentrations of imperatorin in rat liver, kidney, lung, and heart were higher than those in other organs. To determine the free fraction in serum, samples were filtered using ultrafiltration membranes with a molecular weight cut-off of 10 kDa, and extracted using liquid-liquid extraction. The protein binding values in rat plasma, spontaneous hypertensive rat plasma, human plasma and human serum albumin were 84 ± 3, 69 ± 7, 81 ± 7 and 75 ± 3%, respectively. 2009
AB - A rapid and sensitive assay for the quantification of imperatorin in plasma and tissues has been developed. An analysis was performed by gas chromatography/mass spectrometry in the selected ion-monitoring mode. The main pharmacokinetic parameters obtained were T max = 1.23 ± 0.26 h, C max = 0.95 ± 0.38 μg/mL, AUC = 3.42 ± 0.52 h μg/mL and K a = 1.34 ± 0.18 h. The experimental results showed that imperatorin was easily absorbed, but its elimination was slow, from 3 to 12 h after oral administration. The concentrations of imperatorin in rat liver, kidney, lung, and heart were higher than those in other organs. To determine the free fraction in serum, samples were filtered using ultrafiltration membranes with a molecular weight cut-off of 10 kDa, and extracted using liquid-liquid extraction. The protein binding values in rat plasma, spontaneous hypertensive rat plasma, human plasma and human serum albumin were 84 ± 3, 69 ± 7, 81 ± 7 and 75 ± 3%, respectively. 2009
UR - https://www.scopus.com/pages/publications/68249144579
U2 - 10.2116/analsci.25.869
DO - 10.2116/analsci.25.869
M3 - 文章
C2 - 19609025
AN - SCOPUS:68249144579
SN - 0910-6340
VL - 25
SP - 869
EP - 873
JO - Analytical Sciences
JF - Analytical Sciences
IS - 7
ER -