Determination of trenbolone acetate in cattle feeds by a flow injection chemiluminescence method

A flow injection chemiluminescence (CL) method is described for the determination of trenbolone acetate based on the CL generated during its reaction with KMnO ₄ in acidic medium. The CL intensity is greatly enhanced by alizarin yellow R. The CL intensity is linear with trenbolone acetate concentration in the range 0.1-100.0 mg L ^-1 with a detection limit of 0.05 mg L ^-1. The sample throughout is about 90 h ^-1 and the relative standard deviation for 2.0 mg L ^-1 trenbolone acetate solution is 1.5% (n=11). The proposed method was applied to the determination of trenbolone acetate in cattle feeds.