Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry

Yongchuan Gu; Guangji Wang; J. Paul Fawcett

doi:10.1016/j.jchromb.2003.11.033

Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry

Yongchuan Gu
, Guangji Wang
, J. Paul Fawcett

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Astragaloside IV (AGS-IV) is an active constituent of Radix Astragali used in many Traditional Chinese Medicines. This paper describes a sensitive and specific assay for the quantitation of AGS-IV in rat plasma. After solid phase extraction (SPE), samples were analyzed by liquid chromatography electrospray ionization mass spectrometry using a reversed-phase C18 column. The assay was linear in the range 1-500ng/ml with a limit of detection of 0.5ng/ml. The recovery was 92.5% and within-day and between-day precision were 3.7-6.0 and 2.8-9.8%, respectively. The assay was applied to a pharmacokinetic study in rat after a single oral dose. The drug was rapidly absorbed and subsequently eliminated according to a biphasic concentration-time curve.

Original language	English
Pages (from-to)	285-288
Number of pages	4
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	801
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2003.11.033
State	Published - 5 Mar 2004
Externally published	Yes

Keywords

Astragaloside IV

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10.1016/j.jchromb.2003.11.033

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title = "Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry",

abstract = "Astragaloside IV (AGS-IV) is an active constituent of Radix Astragali used in many Traditional Chinese Medicines. This paper describes a sensitive and specific assay for the quantitation of AGS-IV in rat plasma. After solid phase extraction (SPE), samples were analyzed by liquid chromatography electrospray ionization mass spectrometry using a reversed-phase C18 column. The assay was linear in the range 1-500ng/ml with a limit of detection of 0.5ng/ml. The recovery was 92.5\% and within-day and between-day precision were 3.7-6.0 and 2.8-9.8\%, respectively. The assay was applied to a pharmacokinetic study in rat after a single oral dose. The drug was rapidly absorbed and subsequently eliminated according to a biphasic concentration-time curve.",

keywords = "Astragaloside IV",

author = "Yongchuan Gu and Guangji Wang and Fawcett, \{J. Paul\}",

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doi = "10.1016/j.jchromb.2003.11.033",

language = "英语",

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T1 - Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry

AU - Gu, Yongchuan

AU - Wang, Guangji

AU - Fawcett, J. Paul

PY - 2004/3/5

Y1 - 2004/3/5

N2 - Astragaloside IV (AGS-IV) is an active constituent of Radix Astragali used in many Traditional Chinese Medicines. This paper describes a sensitive and specific assay for the quantitation of AGS-IV in rat plasma. After solid phase extraction (SPE), samples were analyzed by liquid chromatography electrospray ionization mass spectrometry using a reversed-phase C18 column. The assay was linear in the range 1-500ng/ml with a limit of detection of 0.5ng/ml. The recovery was 92.5% and within-day and between-day precision were 3.7-6.0 and 2.8-9.8%, respectively. The assay was applied to a pharmacokinetic study in rat after a single oral dose. The drug was rapidly absorbed and subsequently eliminated according to a biphasic concentration-time curve.

AB - Astragaloside IV (AGS-IV) is an active constituent of Radix Astragali used in many Traditional Chinese Medicines. This paper describes a sensitive and specific assay for the quantitation of AGS-IV in rat plasma. After solid phase extraction (SPE), samples were analyzed by liquid chromatography electrospray ionization mass spectrometry using a reversed-phase C18 column. The assay was linear in the range 1-500ng/ml with a limit of detection of 0.5ng/ml. The recovery was 92.5% and within-day and between-day precision were 3.7-6.0 and 2.8-9.8%, respectively. The assay was applied to a pharmacokinetic study in rat after a single oral dose. The drug was rapidly absorbed and subsequently eliminated according to a biphasic concentration-time curve.

KW - Astragaloside IV

UR - https://www.scopus.com/pages/publications/1642482451

U2 - 10.1016/j.jchromb.2003.11.033

DO - 10.1016/j.jchromb.2003.11.033

M3 - 文章

C2 - 14751797

AN - SCOPUS:1642482451

SN - 1570-0232

VL - 801

SP - 285

EP - 288

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Determination of Astragaloside IV in rat plasma by liquid chromatography electrospray ionization mass spectrometry

Abstract

Keywords

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