Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes

Nuno M.M. Pires; Tao Dong; Zhaochu Yang; Frank Karlsen

doi:10.1109/EMEIT.2011.6023694

Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes

Nuno M.M. Pires
, Tao Dong
, Zhaochu Yang
, Frank Karlsen

University of South-Eastern Norway

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)₆]^3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.

Original language	English
Title of host publication	Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
Pages	2841-2844
Number of pages	4
DOIs	https://doi.org/10.1109/EMEIT.2011.6023694
State	Published - 2011
Externally published	Yes
Event	2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011 - Harbin, China Duration: 12 Aug 2011 → 14 Aug 2011

Publication series

Name	Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
Volume	6

Conference

Conference	2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011
Country/Territory	China
City	Harbin
Period	12/08/11 → 14/08/11

Keywords

bio-activated microbeads
interdigitated microelectrodes
interfacial impedance sensor
nickel hexacyanoferrate film
pathogen detection

Access to Document

10.1109/EMEIT.2011.6023694

Cite this

Pires, N. M. M., Dong, T., Yang, Z., & Karlsen, F. (2011). Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes. In Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011 (pp. 2841-2844). Article 6023694 (Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011; Vol. 6). https://doi.org/10.1109/EMEIT.2011.6023694

Pires, Nuno M.M. ; Dong, Tao ; Yang, Zhaochu et al. / Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes. Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011. 2011. pp. 2841-2844 (Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011).

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title = "Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes",

abstract = "In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.",

keywords = "bio-activated microbeads, interdigitated microelectrodes, interfacial impedance sensor, nickel hexacyanoferrate film, pathogen detection",

author = "Pires, \{Nuno M.M.\} and Tao Dong and Zhaochu Yang and Frank Karlsen",

year = "2011",

doi = "10.1109/EMEIT.2011.6023694",

language = "英语",

isbn = "9781612840857",

series = "Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011",

pages = "2841--2844",

booktitle = "Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011",

note = "2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011 ; Conference date: 12-08-2011 Through 14-08-2011",

}

Pires, NMM, Dong, T, Yang, Z & Karlsen, F 2011, Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes. in Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011., 6023694, Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011, vol. 6, pp. 2841-2844, 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011, Harbin, China, 12/08/11. https://doi.org/10.1109/EMEIT.2011.6023694

Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes. / Pires, Nuno M.M.; Dong, Tao; Yang, Zhaochu et al.
Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011. 2011. p. 2841-2844 6023694 (Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011; Vol. 6).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes

AU - Pires, Nuno M.M.

AU - Dong, Tao

AU - Yang, Zhaochu

AU - Karlsen, Frank

PY - 2011

Y1 - 2011

N2 - In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.

AB - In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.

KW - bio-activated microbeads

KW - interdigitated microelectrodes

KW - interfacial impedance sensor

KW - nickel hexacyanoferrate film

KW - pathogen detection

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T3 - Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011

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BT - Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011

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ER -

Pires NMM, Dong T, Yang Z, Karlsen F. Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes. In Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011. 2011. p. 2841-2844. 6023694. (Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011). doi: 10.1109/EMEIT.2011.6023694

Detection of pathogens using bio-activated nickel hexacyanoferrate film coated interdigitated microelectrodes

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