Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

Rui Zhou; Pan Liu; Xiaokang Peng; Li Tang; Yanmin Zhang; Xiaoguai Liu

doi:10.1016/j.scr.2026.103927

Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

Rui Zhou
, Pan Liu
, Xiaokang Peng
, Li Tang
, Yanmin Zhang
, Xiaoguai Liu

Health Science Center

Xi’an Children’s Hospital
Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

Abstract

We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

Original language	English
Article number	103927
Journal	Stem Cell Research
Volume	92
DOIs	https://doi.org/10.1016/j.scr.2026.103927
State	Published - Apr 2026

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10.1016/j.scr.2026.103927

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title = "Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome",

abstract = "We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.",

author = "Rui Zhou and Pan Liu and Xiaokang Peng and Li Tang and Yanmin Zhang and Xiaoguai Liu",

note = "Publisher Copyright: {\textcopyright} 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/",

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AU - Zhou, Rui

AU - Liu, Pan

AU - Peng, Xiaokang

AU - Tang, Li

AU - Zhang, Yanmin

AU - Liu, Xiaoguai

PY - 2026/4

Y1 - 2026/4

N2 - We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

AB - We developed a well-characterized induced pluripotent stem cell (iPSC) line. This line was generated by reprogramming peripheral blood mononuclear cells from a male Alagille Syndrome baby using the Sendai viral method, which maintains genomic stability and pluripotent capacity. Markers of undifferentiated iPSCs were validated via immunocytochemistry. Trilineage potential was confirmed through immunofluorescent detection of lineage-specific markers (endoderm, mesoderm, ectoderm). Karyotypic analysis verified a normal diploid complement (46, XY). Additional assessments demonstrated Sendai virus clearance and absence of mycoplasma contamination. This iPSC line serves as a robust resource for disease modeling and regenerative medicine research.

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SN - 1873-5061

VL - 92

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 103927

ER -

Derivation of an iPSC line using the Sendai virus from a newborn boy with Alagille Syndrome

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