TY - GEN
T1 - Controllable olfactory cellular network formation on polyaniline conducting polymer modified microelectrode array
AU - Du, Liping
AU - Li, Yu
AU - Wang, Jian
AU - Qin, Zhen
AU - Wu, Chunsheng
AU - Wang, Ping
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/5
Y1 - 2017/7/5
N2 - Here a conducting polymer-based cell immobilization method was presented to form controllable olfactory cellular network on microelectrode array. PAIN conducting polymer was electropolymerized on the surface of microelectrode array in a sequence in order to modify the defined microelectrode. Different antibodies can be linked covalently onto defined sites for the effective capture and immobilization of different cells. Based on the specific recognition between antibody and cells, olfactory receptor cells were site-specifically immobilized on the surface of microelectrodes. The fluorescent staining results indicate that olfactory cells were captured effectively with the microelectrode array. Extracellular potential changes can be efficiently monitored from olfactory cells in response to odorant stimulations. All the results demonstrated that this immobilization method can be a universal method to control the arrangement of cells on the sensor surface, which have great application prospect in the field of cell-based biosensor.
AB - Here a conducting polymer-based cell immobilization method was presented to form controllable olfactory cellular network on microelectrode array. PAIN conducting polymer was electropolymerized on the surface of microelectrode array in a sequence in order to modify the defined microelectrode. Different antibodies can be linked covalently onto defined sites for the effective capture and immobilization of different cells. Based on the specific recognition between antibody and cells, olfactory receptor cells were site-specifically immobilized on the surface of microelectrodes. The fluorescent staining results indicate that olfactory cells were captured effectively with the microelectrode array. Extracellular potential changes can be efficiently monitored from olfactory cells in response to odorant stimulations. All the results demonstrated that this immobilization method can be a universal method to control the arrangement of cells on the sensor surface, which have great application prospect in the field of cell-based biosensor.
KW - Cell-based biosensor
KW - Conducting polymer
KW - Controllable immobilization
KW - Microelectrode array
KW - Olfactory sensory cell
UR - https://www.scopus.com/pages/publications/85027218050
U2 - 10.1109/ISOEN.2017.7968910
DO - 10.1109/ISOEN.2017.7968910
M3 - 会议稿件
AN - SCOPUS:85027218050
T3 - ISOEN 2017 - ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, Proceedings
BT - ISOEN 2017 - ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2017 ISOCS/IEEE International Symposium on Olfaction and Electronic Nose, ISOEN 2017
Y2 - 28 May 2017 through 31 May 2017
ER -