Abstract
Objective: To construct an overexpression vector of microR-338-3p (miR-338-3p) and detect its effect on the proliferation of human gastric cancer cell line SGC-7901.
Methods: pcDNATM6.2-GW/EmGFP-miR eukaryotic expression vector was cut using restriction site of HindIII and EcoRI, connected to miR-338-3p oligonucleotide, and identified by sequencing and blast. miR-338-3p overexpression vector was transfected into SGC-7901 cells and the expression level was determined by real-time PCR. The proliferation of SGC-7901 cells was also examined by MTT.
Results: The overexpression vector of miR-338-3p was identified as real by sequencing. The expression level of miR-338-3p in SGC-7901 cells with expression vectors transfected was increased significantly (P<0.05). The proliferation of SGC-7901 cells in vitro was also inhibited.
Conclusion: The overexpression vector of miR-338-3p was successfully constructed and human gastric cancer cell SGC-7901 instantaneously expressing miR-338-3p was obtained, which preliminarily confirmed that the overexpression of miR-338-3p can inhibit the proliferation of cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 442-446 |
| Number of pages | 5 |
| Journal | Journal of Xi'an Jiaotong University (Medical Sciences) |
| Volume | 35 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1 Jul 2014 |
Keywords
- Cell proliferation
- Expression vector
- Gastric cancer
- miR-338-3p
