Construction of eukaryotic expression vector of wtp53/junB fusion gene

Objective: To construct wtp53/junB fusion gene and its eukaryotic expression vector in order to provide the basis for further application of polygene union therapy in hepatocellular carcinoma. Methods: Polymerase chain reaction (PCR), reverse transcription-PCR (RT-PCR) and gene recombination techniques were used to construct the eukaryotic vector of pEGFP-Cl-wtp53/junB fusion gene, which carries the enhanced green fluorescent protein (EGFP). The transfection of pEGFP-Cl-wtp53/junB in hepatoma HepG2 cells was detected by the location of green fluorescence. Results: The DNA sequence of wtp53/junB fusion gene was successfully cloned into the pEGFP-Cl plasmid and the sequence was the same as what we expected. Green fluorescence located on cell nucleus proved that pEGFP-Cl-wtp53/junB was transfected into HepG2 cell line successfully. Conclusion: We successfully constructed the eukaryotic vector of pEGFP-Cl-wtp53/junB fusion gene, which carries the EGFP, and transfects it into human hepatoma cell nucleus. It may lay the basis for studying the synergetic effect of wtp53 and junB in hepatocellular carcinoma.