Construction of a recombinant expression vector of rgpAcd gene of porphyromonas gingivalis

Objective: To construct prokaryotic expression vector of the catalytic domain of rgpAcd of porphyromonas gingivalis (Pg). Methods: The desired DNA fragment rgpAcd was obtained by PCR and was separately sequenced and identified by inserting into inter-vector pMD18-T Vector. The correct fragment was linked with and cloned into an prokaryotic expression vector pET-15b. Results: A 1 476 bp specific fragment was obtained and DNA sequencing showed that the fragment was consistent with those of the published. After the recombinant expression plasmid was confirmed by enzymes digestion, it showed that the prokaryotic expression vector of rgpAcd gene was constructed successfully. Conclusion: The protein of rgpAcd will be obtained for further study. Successful construction of live attenanated vaccine candidate plasmid lays solid foundation for future animal experiments and clinical trials.